Sentences with phrase «agarose gel containing»
PCR products were visualized by blue light after electrophoresis on a 2.5 % agarose gel containing 1 × GelStar ® Nucleic Acid Gel Stain (Lonza, Breda, The Netherlands) in 1 × Tris - borate buffer (pH 8.0).
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The amplified DNA products were analyzed on a 1.0 % agarose gel, and pieces of gel containing the amplified DNA bands were excised and purified using a gel extraction kit (Takara Bio).