Amplicons were visualized in a 2 %
agarose gel stained with Ethidium Bromide and purification was performed directly from the amplification reaction using the Qiagen PCR purification Kit according to the manufacturer's instructions.
PCR products were visualized by blue light after electrophoresis on a 2.5 %
agarose gel containing 1 × GelStar ® Nucleic Acid Gel Stain (Lonza, Breda, The Netherlands) in 1 × Tris - borate buffer (pH 8.
gel containing 1 × GelStar ® Nucleic Acid
Gel Stain (Lonza, Breda, The Netherlands) in 1 × Tris - borate buffer (pH 8.
Gel Stain (Lonza, Breda, The Netherlands) in 1 × Tris - borate buffer (pH 8.0).