Sentences with phrase «aliquots from»

Equal aliquots from individual CSF samples were pooled to provide sufficient volume for extensive fractionation and two - dimensional LC coupled to tandem MS (2D - LC - MS / MS) analysis with immunoaffinity depletion from 30 women and 13 men (n = 43) who fulfilled the 1994 case definition for CFS [1].

Equal aliquots from individual CSF samples were pooled to provide sufficient volume for extensive fractionation and 2D - LC - MS / MS analysis with immunoaffinity depletion from 15 females and 10 males (n = 25) with nPTLS.

A 0.1 mL aliquot from any culture exhibiting CPE was passed to fresh monolayers and the remainder of the sample was harvested for an isolate and confirmation of POWV presence, regardless of lineage, by qRT - PCR.

Not exact matches

Once no longer needed clinically, aliquots were cultured for bacteria.In total, 30 bottles were returned for culture; six were excluded from analysis because human milk fortifier had been added, and two had been left out of the refrigerator.

A 10 mL aliquot of fresh milk (mature milk) was collected from a complete expression of the left side at least two hours since the previous feeding.

Western blots were performed using 20 µg aliquots of protein from fetal or adult hearts, resolved on 10 % Tris - glycine - SDS polyacrylamide gels (Bio-Rad, Hertfordshire, UK) and immobilised on PVDF membranes.

20 µL aliquots were transferred to a 384 - well PCR plate and scanned at a heating rate of 1 °C / min from 20 to 95 °C.

An aliquot of coded plasma from the CFS and healthy controls was tested at RKI by an ELISA using recombinant Gag and Env proteins used recently to investigate XMRV infection in German prostate cancer patients [14].

Results from each aliquot were plotted by the software onto a simultaneously run calibration curve generated from known quantities of DYS14.

Interestingly, in the current study, we were unable to recover sequence from this related MLV in a fresh aliquot of 2003 LnCaP cells by deep sequencing, and instead, found only XMRV (Fig. 5; Fig.

In brief, six aliquots of DNA from each specimen were tested for DYS14.

To investigate this possibility, aliquots of 2003 LNCaP cells from the Cleveland Clinic laboratory and 2012 LNCaP cells from the UCSF Cell Culture Facility were tested for XMRV by gag RT - PCR (Fig. 1).

The medication they received from their veterinarian was not dispensed in the CVM - approved container but was broken into aliquots that were taken home without the client information sheet or approved label.