Sentences with phrase «aliquots of»

«We store aliquots of cells for each individual patient and will evaluate the efficacy of the adult stem cell transplantation into the dilated, failing myocardium of DCM dogs.»

Aliquots of the 38 plasma samples collected during each 24 - h GCRC admission were pooled as described in Subjects and Methods for measurement of average hourly plasma ghrelin concentrations.

To investigate this possibility, aliquots of 2003 LNCaP cells from the Cleveland Clinic laboratory and 2012 LNCaP cells from the UCSF Cell Culture Facility were tested for XMRV by gag RT - PCR (Fig. 1).

In brief, six aliquots of DNA from each specimen were tested for DYS14.

2 µl aliquots of the purified PCR product were used for sequencing PCR with BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems).

Ten - microliter aliquots of the nested PCR products were subjected to RFLP analysis by digestion with 2 U of either HinfI or MseI, and digested fragments were resolved by agarose gel electrophoresis in TBE buffer, as described elsewhere [19, 28].

Protein content was measured on aliquots of extract.

Western blots were performed using 20 µg aliquots of protein from fetal or adult hearts, resolved on 10 % Tris - glycine - SDS polyacrylamide gels (Bio-Rad, Hertfordshire, UK) and immobilised on PVDF membranes.

Two aliquots each of purified plasmid DNA's containing envelope sequence subtype refrenceThe plasmids in this kit are for use in HMA only.

Injecting stock solution aliquots of the catalyst and reactants into a carrier solvent stream let the authors vary the main solvent efficiently and scale up the optimal conditions for product isolation.

After written informed consent, these participants provided a single, 10 mL aliquot of freshly expressed milk (mature milk).

A 10 mL aliquot of fresh milk (mature milk) was collected from a complete expression of the left side at least two hours since the previous feeding.

Additionally, an aliquot of 4 × 106 splenocytes was stained for Foxp3 expression using methods and reagents, provided by the manufacturer (eBioscience), prior to flow cytometric analyses.

An aliquot of 50 μl was removed for protein determination (11).

A 0.1 - mL aliquot of this suspension was introduced into 6 mL of BSK II medium supplemented with 6 % rabbit serum, 1.2 % gelatin, and 35 % liquid bovine serum albumin (Sigma, St. Louis)(complete BSK II) and was incubated at 33 °C for ⩽ 8 weeks.

An aliquot of coded plasma from the CFS and healthy controls was tested at RKI by an ELISA using recombinant Gag and Env proteins used recently to investigate XMRV infection in German prostate cancer patients [14].

All procedures will be tested by thawing a small aliquot of embryos and determining survival.

PCR was performed by adding a 2 - μl aliquot of each sample to the PCR mix (Quantimix Easy Sig Kit; Biotools) containing the specific primers.

Interestingly, in the current study, we were unable to recover sequence from this related MLV in a fresh aliquot of 2003 LnCaP cells by deep sequencing, and instead, found only XMRV (Fig. 5; Fig.

A 100 - μl aliquot of the buffer was used for the lipase assay in combination with 100 μl of a 10 % Intralipid / [3H] triolein emulsion for 1 hour at 25 °C.

(d) In addition, if the test fails to reconfirm the presence of the drug (s) / drug metabolite (s) reported in the primary specimen, you may send the specimen or an aliquot of it for testing at another HHS - certified laboratory that has the capability to conduct another reconfirmation test.

(b) In addition, if the test fails to reconfirm the adulterant result reported in the primary specimen, you may send the specimen or an aliquot of it for testing at another HHS - certified laboratory that has the capability to conduct another reconfirmation test.

It takes with it an aliquot of thicker ice.

Not exact matches

Once no longer needed clinically, aliquots were cultured for bacteria.In total, 30 bottles were returned for culture; six were excluded from analysis because human milk fortifier had been added, and two had been left out of the refrigerator.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients.

Health and Medicine Reporter Research Alexander Zaika Aliquots Department of Veterans Affairs digestive disease research center esophageal cancer Gastroesophageal reflux disease GERD NCI NIDDK NIH reactive oxygen species Reporter Sept 15 2017 Scientific Reports Vanderbilt Ingram Cancer Center

Health and Medicine Reporter Research Aliquots American Heart Association cell and developmental biology cell division contractile ring Human Frontier Science Program Journal of Cell Biology kathleen gould lipid NIGMS NIH Reporter Nov 10 2017

Health and Medicine Reporter Research Aliquots Andrea Page - McCaw Department of Cell and Developmental Biology development NICHD NIGMS NIH Reporter Feb 23 2018 stem cell stem cell research stem cells Wnt signaling

Health and Medicine Reporter Research Aliquots American Journal of Respiratory Critical Care Medicine genetics inherited disease James Loyd John Newman journal publication medicine NCATS NHLBI NIH pediatrics pulmonary hypertension Reporter Nov 16 2012 Vanderbilt Institute for Medicine and Public Health

20 µL aliquots were transferred to a 384 - well PCR plate and scanned at a heating rate of 1 °C / min from 20 to 95 °C.

The cell culture supernatant was collected and concentrated if necessary, and then 100 μL aliquots were used for detection with ELISA kit (PBL Biomedical Laboratories) following the protocol of the manufacturer.

Results from each aliquot were plotted by the software onto a simultaneously run calibration curve generated from known quantities of DYS14.

Health and Medicine Reporter Research Aliquots blindness blood vessel COX - 2 Department of Biochemistry imaging Jashim Uddin Journal of Biomedical Optics Lawrence Marnett NCI NEI NIH Reporter Oct 7 2016 retinal degeneration

Health and Medicine Reporter Research Aliquots American Heart Association BMPR2 clinical trial Department of Medicine estrogen European Respiratory Journal Gilead Sciences James West NHLBI NIH pulmonary arterial hypertension Reporter Aug 18 2017 tamoxifen

Health and Medicine Reporter Research Aging and Mental Health Aliquots Alzheimer's disease CTSA Department of Psychiatry depression NIA NIH pain Reporter Sept 1 2017 School of Nursing Todd Monroe

A 0.1 mL aliquot from any culture exhibiting CPE was passed to fresh monolayers and the remainder of the sample was harvested for an isolate and confirmation of POWV presence, regardless of lineage, by qRT - PCR.

These aliquots were used to initiate blood infections in naïve mice that were then used as donors of asexual parasites to mice involved in the posttransmission experiments.

The luminescence dating of the Monte Verde sediments was based on the Single - Aliquot Regenerative (SAR) dose protocol [40](see Text G in S1 File) applied to concentrates of quartz and K - rich feldspar in the 180 — 250 μm grain size.

Samples in which only 1 or 2 of the triplicate aliquots were positive could only be considered presumptively positive.

Our storage system enables retrieval of up to 1.2 million one - milliliter aliquots at any time.

Fecal samples were frozen within 12 hours after collection and were pooled for analysis of small aliquots.

If I was a representative of the CDC, took 40,000 blood samples, split them into 7 aliquots, then mailed them to seven different labs and they all reported that 0.56 % were positive for syphilis, but all reported completely regional distributions of STD infection, it wouldn't be something to worry about?

Helped design and carry out experimental procedures Performed Immunochemical staining Prepared Media and aliquots Conducted basic biological manipulations of bacteria Performed cell culture and seeding Participated in the Department's Quality Control, Quality Assurance, Safety, and preventive maintenance programs.