When these building blocks interact with a cervical cancer biomarker known as glycosylphosphatidylinositol - anchored placental
alkaline phosphatase, or GPI - anchored PLAP,
which is located on lipid rafts, they self - assemble into nano - scale chains, or «nanofibrils», that glue lipid rafts into large clusters.
Since
alkaline phosphatase, coupled to the secondary antibody for the BrdU primary antibody, may have complicated the interpretation of the wounding experiments, we also treated human corneal - limbal tissues with BrdU histochemical assays in
which fluorescein, rather than
alkaline phosphatase, was coupled to the secondary antibody.
That, in turn, activates an inducible reporter gene,
which expresses a secreted form of
alkaline phosphatase.
When five lines were further expanded and characterized, all demonstrated expression of key pluripotency markers expressed by hESCs,
which included:
alkaline phosphatase, Nanog, SSEA3, SSEA4, TRA160 and TRA181 (Figure 5A).
Other standout lots were the very strong «Self Portait as a Heel - Part Two,» by Jean - Michel Basquiat (1960 - 1988), Lot 16, shown at the top of this article, an acyrilic and oil paintstick on canvas, 1982, that sold for $ 772,500, well above its high estimate of $ 600,000; two sculptures by Agnes Martin (b. 1912), Lots 17 and 18,
which sold for $ 217,000 and $ 233,500, respectively, both considerably over their respective high estimates of $ 45,000 and $ 120,000; «Broadway and 64th Street,» a very good, large oil by Richard Estes (b. 1932), that sold above its $ 300,000 high estimate for $ 354,500; Lot 31, a large canvas from his Rorschach series by Andy Warhol (1928 - 1987) that more than doubled its high estimate and sold for $ 684,500; Lot 13, «
Alkaline Phosphatase - Polyethelene Glycol,» by Damien Hirst (b. 1965), 1992, sold for $ 140,000 and had a high estimate of $ 80,000; and Lot 37, «a delightful large acrylic on vinyl tarpaulin by Keith Haring (1958 - 1990) that sold for $ 200,500 and had a high estimate of $ 150,000.