Sentences with word «alloantigen»

Negative controls consisted of naïve C57BL / 6 mice that had been exposed to BALB / c alloantigens in vivo.

This is in keeping with previous reports indicating that orthotopic corneal allografts in mice do not normally induce detectable CTL responses to donor alloantigens in normal mice and corneal allograft rejection occurs in CD8 KO mice and perforin KO mice, which can not generate allospecific CTL (8, 9).

It was shown that chronically stimulated T cells proliferating in response to alloantigen in the context of GVHD greatly increased both glycolysis and mitochondrial oxidative phosphorylation compared with normal T cells (51).

There is controversy surrounding the cell surface expression of MHC alloantigens by MSC.

University of Texas M.D. Anderson Cancer Center Tumor - specific alloantigen - anergic donor - derived T - cell therapy after hematopoietic stem - cell transplantation

Thus, the frequency of alloreactive T cells towards the donor type alloantigen was the same in both tolerant and non-tolerant animals.

Eva Guinan, M.D. Harvard Medical School Dana Farber Cancer Institute Extending the donor pool by inducing alloantigen specific T - cell anergy ex vivo for human hematopoietic stem cell transplantation

Like wild - type mice, C57BL / 6 CD4 KO mice developed DTH responses to BALB / c alloantigens following rejection of their BALB / c corneal allografts (data not shown).

A small number of in - vivo studies suggest that MSC play a role in enabling alloantigen tolerance.

Maternal Alloantigens Promote the Development of Tolerogenic Fetal Regulatory T Cells in utero.

Responses to third - party Balb / c mice were similar in tolerant, sensitized and control non-transplanted mice showing specificity to donor - type alloantigen as would be expected.

Additional experiments were performed to determine if both CD8 + and CD8 − T lymphocytes from CD4 KO mice that had rejected BALB / c corneal allografts were capable of mounting DTH responses to BALB / c alloantigens.

By contrast, DN T cells are able to mediate DTH to donor alloantigens and induce apoptosis of donor - specific corneal endothelial cells.

Interestingly, recipients of CD8 + T lymphocytes failed to mount DTH responses to BALB / c alloantigens (Figure 4), even though similar panels of mice rejected 95 % of their BALB / c corneal allografts (Figure 2B).

Lymphoid cells were adoptively transferred to C57BL / 6 beige nude mice and DTH responses to BALB / c alloantigens were determined 24 hr later using a conventional footpad swelling assay.

Development of DTH responses to donor alloantigens has been correlated with corneal allograft rejection.

By contrast, recipients of CD8 − T lymphocytes from CD4 KO donors developed positive DTH responses to BALB / c alloantigens that were significantly greater than the negative controls and the recipients of CD8 + T lymphocytes (P = 0.01).

MSCs can suppress the T lymphocyte proliferation induced by alloantigens, mitogens and anti-CD3 and anti-CD28 antibodies in vitro, in humans, baboons and mice 12, 13, 14 15, 16, 17, 18.

The major limit to solid organ graft survival is T - cell recognition by the recipient of alloantigen (dominated by, but not confined to MHC / HLA antigens) 71.

Previous exposure to alloantigens is one of the major differences between clinical patients and most experimental models of transplantation.

Additionally, splenocytes of recipient animals were used as responder cells in mixed lymphocyte reactions and ELISPOT to determine responses to donor alloantigen.

Although the T cells of chimeric animals responded to alloantigen, the response was similar to that of non-chimeric control animals, suggesting that these cells did not appear to lead to deletion of alloreactive T cells.

Once long - term mixed chimerism was established, we determined the influence of donor cells on the ability of the host's T cells to respond to alloantigen.