Allogenic MRL and control syngeneic 129SvJ mice were sublethally irradiated and subsequently transplanted HPCs.
Since our flow cytometric data had shown that the HPCs poorly expressed MHC antigens [17], we wondered whether they stimulate
allogenic MRL splenocytes (H2k).
We therefore transplanted Rag2 − / − γc − / − mice,
allogenic MRL mice and syngeneic 129SvJ mice with 2 × 106 HPCs after sublethal irradiation.
Not exact matches
To determine whether HPCs stimulate
allogenic T cells,
MRL splenocytes were co-cultured with irradiated 129SvJ splenocytes, HPCs, or bone marrow cells.
Despite the
allogenic barrier, the
MRL mice showed a similar pattern as the syngeneic recipients, suggesting that the engraftment was less likely to be regulated by adaptive immunological factors.