Sentences with phrase «alpha tubulin»

Another post-translational modification of detyrosinated alpha tubulin is C - terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

The cells were then incubated with the antibody ab16056 at 1µg / ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4 °C.

The cells were then incubated with ab7291 at a working concentration of 0.5 μg / ml and ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin (Alexa Fluor ® 647, shown in red) at 1/250 overnight at +4 °C, followed by a further incubation at room temperature for 1h with an anti-mouse AlexaFluor ® 488 (ab150117) at 2 μg / ml (shown in green).

Ab7291 staining alpha tubulin in human breast cancer cell line by ICC / IF (Immunocytochemistry / Immunofluorescence).

Alpha tubulin is not suitable as a loading control in adipose tissue as expression of tubulin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29 (1): 53 - 60, «in cells undergoing adipose differentiation actin synthesis decreases by 90 %»).

Detyrosinated alpha tubulin is referred to as Glu - tubulin.

Alpha tubulin (red) was detected using the mouse monoclonal (ab7291) antibody.

ab7291 staining alpha Tubulin in SV40LT - SMC cells.

IHC image of ab7291 staining alpha Tubulin in rat colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond.

Tyrosine ligase adds a C - terminal tyrosin to monomeric alpha tubulin.

Not exact matches

Microtubules are hollow cylinders with walls made up of tubulin proteins — alpha (green) and beta (blue)-- plus EB proteins (orange) that can either stabilize or destabilize the structure of the tubulin proteins.

The energy maybe required to build stable alpha and beta tubulin assembly.

Building from two subunits, alpha and beta tubulin, this protein assembles into microtubules that play a vital role inside cells — giving structure, pushing or pulling other things around, or providing a track on which other molecules can pull themselves along.

Assembling alpha - beta tubulin dimers involves six known genes, Al - Bassam said.

Microtubule strands assemble from subunits of alpha and beta tubulin proteins that form dimers.

Most importantly, they found that the active site of TTLL7 is ideally positioned to contact the negatively charged «beta - tail» of beta - tubulin, one of the two protein building blocks of the microtubule polymer (alpha - and beta - tubulin).

Immunofluorescent imaging of human cells (U2OS) with ab7291 reveals a delicate network of alpha - tubulin (green) located exclusively in the cytoplasm.

An alpha and beta tubulin heterodimer is the basic structural unit of microtubules.

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon.

The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical.

ab7291 staining alpha - Tubulin in Caco - 2 cells.

Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tuTubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulintubulin).

GAD, myelin basic protein, asialoganglioside, alpha and beta tubulin, cerebellar, synapsin: Neurological autoimmunity