Sentences with phrase «alternative isoforms»

However, although many thousands of alternatively spliced transcripts are routinely detected in RNA - seq studies, reliable large - scale mass spectrometry - based proteomics analyses identify only a small fraction of annotated alternative isoforms.

The clearest finding from proteomics experiments is that most human genes have a single main protein isoform, while those alternative isoforms that are identified tend to be the most biologically plausible: those with the most cross-species conservation and those that do not compromise functional domains.

As a proxy for splicing alteration, we computed the Shannon's entropy on the relative abundance of a gene's alternative splicing isoforms (Methods)-- higher values corresponding to more stochastic production of alternative isoforms.

Not exact matches

However, its extreme length (> 13kb), number of exons (> 50) and high level of alternative splicing means CACNA1C isoform structure and expression is poorly understood, making it difficult to identify which gene isoforms confer disease risk and where they are expressed.

(D) Diagrammatic image of four transcript isoforms that were detected as alternative splice variants resulting from a SNP in the 2nd intron of bsl1 - 2.

The usage of a major isoform across tissues and biological conditions has been reported as a general characteristic of genes38, 39, and has lead to extensive debate on the physiological relevance of regulated alternative splicing39.

More than half of mammalian genes generate multiple messenger RNA isoforms that differ in their 3 ′ untranslated regions (3 ′ UTRs) and therefore in regulatory sequences, often associated with cell proliferation and cancer; however, the mechanisms coordinating alternative 3 ′ - UTR processing for specific mRNA populations remain poorly defined.