Sentences with phrase «analysis of a single gene»

To make BinoX directly usable for other researchers, a public web server http://PathwaX.sbc.su.se (Ogris et al., 2016b) was set up for on - line pathway analysis of single gene sets, which applies the BinoX algorithm to all KEGG pathways and FunCoup networks.

Not exact matches

Their preliminary analysis revealed several mutations known as single nucleotide polymorphisms (SNPs) in the ALR gene, many of which haven't been identified before.

Working with this hypothesis, the researchers conducted a statistical analysis of the CX3CR1 gene in over 7000 schizophrenia and autism patients and healthy subjects, finding one mutant candidate, a single amino acid switch from alanine to threonine, as a candidate marker for prediction.

Unlike other autoimmune disorders with associated risk factors, APECED is clearly caused by a mutation in a single gene, based on analyses of affected families.

Using a combination of old - fashioned clinical observation and modern biochemical analysis, he has shown that a person's appetite and their eating behavior can be linked to specific genes — and that even a tiny defect such as the absence of a single nucleic acid in a sequence of DNA can lead to runaway weight gain.

The tool acts with surgical precision to replace only abundances that have most likely dropped out and can be used in any type of single - cell gene - expression analysis.

Results from a statistical analysis shows a cluster of SNPs — single nucleotide polymorphisms — in one section of a single gene, indicating the location of a mutation likely linked to autism.

Labs have started tackling the problem with a tool called single - cell transcriptome analysis, which gives readouts of all the genes that are active in individual cells.

Single - cell differential gene expression analysis revealed a spectrum of known transcripts, including several linked to cytotoxic and costimulatory function that are expressed at higher levels in the TEMRA (effector memory T cells expressing CD45RA) subset, which is highly enriched for CD4 - CTLs, compared with CD4 + T cells in the central memory (TCM) and effector memory (TEM) subsets.

He has worked in the biotech industry as a research scientist for over 11 years with a focus on emerging technologies including gene targeting in mice, molecular analysis of transgenes using GFP variants at the single cell level, and developing flow cytometry reagent kits to speed up assay development time for researchers.

Further analysis revealed that a single - letter change in the gene accounted for 46 per cent of the population's hair colour variation, with the blonde allele being recessive to the dark hair allele.

Typically, genome analysis studies primarily look for single changes — one altered unit of DNA — not wholesale copying or halving of genes.

This single cell transcriptome analysis followed by computational analyses enabled the team to identify the gene expression profiles of cells in the process of changing from ES cells to 2CLCs.

Previous genetic studies have examined the association of aspirin, NSAIDs, or both with colorectal cancer according to a limited number of candidate genes or pathways.6 - 10 Thus, to comprehensively identify common genetic markers that characterize individuals who may obtain differential benefit from aspirin and NSAIDs, we conducted a discovery - based, genome - wide analysis of gene × environment interactions between regular use of aspirin, NSAIDs, or both and single - nucleotide polymorphisms (SNPs) in relation to risk of colorectal cancer.

Technological advances in genetic analysis have uncovered changes in single genes that account for a surprising number of infantile and early - childhood epilepsies.

This has led to the analysis of single cell gene expression - ie which genes are switched on in a cell.

Sequencing accelerates the analysis of cancer associated alterations in genome sequence such as insertions and deletions, CNVs, inversions, duplications, translocations and gene fusions, as well as single nucleotide variants.

Topics for the scientific sessions are: Single cell genomics and gene expression Genetic interactions RNAi and somatic cell genetics Protein - DNA interactions Cancer The meeting also highlights existing opportunities for academic and industrial researchers to access automated cell - based and biochemical technologies based at the Karolinska High Throughput Center; home to one of the most sophisticated, state - of - the - art, ultra-high performance liquid handling and analysis platforms in Europe.

Analysis of a linkage peak on chromosome 13 with a dense set of SNPs (single nucleotide polymorphisms) revealed a four - SNP haplotype spanning the gene encoding FLAP (ALOX5AP) that confers an approximately twofold increased risk of myocardial infarction, or heart attack.

Bethesda, USA (2016 - present) Research areas: Super-resolution microscopy, single - molecule imaging, gene expression, computational modeling and data analysis This section includes all projects during my postdoctoral research stay at the National Institutes of Health in Bethesda, MD (Unites States): (9) Understanding gene expression in eukaryotic cells»

After careful analysis of over 80,000 ESTs to find full - length cDNA clones, including verification of the 3» ends of these clones, we have selected 5,849 non-redundant cDNA clones for single colony purification and re-arraying (see What is the Drosophila Gene Collection?

We are using live cell imaging, single cell analysis and gene editing to resolve the molecular determinants of human epidermal progenitor cell division outcomes (two progenitors, two differentiating cells or one or each) and the mode of cell division (maintenance mode vs repair mode).

Comparison of mRNA and protein expression revealed distinct expression profiles for many genes and underscores the importance of multi-omic analysis in single cells.

The researchers believe noise could play a role, so they combine single - molecule analysis with time - lapse movies of individual cells to track random activity in many different genes as the cells switch states.

In the first paper, utilizing genomic analysis of nearly 8,500 Icelandic and Dutch participants, the deCODE team identified a novel, tightly - linked pair of single - letter variants (SNPs) near the ASIP (agouti signaling protein) gene on chromosome 20 that greatly increase the likelihood of an individual being prone to freckles and sunburn.

Another common theme identified in the analysis revealed that students did not fully understand concepts related to heredity and patterns of inheritance (14 %); these essays reflected studentsâ $ ™ belief that single genes are the cause of traits and inherited diseases.

This section invites manuscripts describing (a) Linkage, association, substitution or positional mapping and epigenetic studies in any species; (b) Validation studies of candidate genes using genetically - engineered mutant model organisms; (c) Studies focused on epistatis and gene - environment interactions; (d) Analysis of the functional implications of genomic sequence variation and aim to attach physiological or pharmacogenomic relevance to alterations in genes or proteins; (e) Studies of DNA copy number variants, non-coding RNA, genome deletions, insertions, duplications and other single nucleotide polymorphisms and their relevance to physiology or pharmacology in humans or model organisms, in vitro or in vivo; and (f) Theoretical approaches to analysis of sequence vaAnalysis of the functional implications of genomic sequence variation and aim to attach physiological or pharmacogenomic relevance to alterations in genes or proteins; (e) Studies of DNA copy number variants, non-coding RNA, genome deletions, insertions, duplications and other single nucleotide polymorphisms and their relevance to physiology or pharmacology in humans or model organisms, in vitro or in vivo; and (f) Theoretical approaches to analysis of sequence vaanalysis of sequence variation.

Our analysis of 23,184 cells identifies 15 clusters, few of which could be fully characterised by a single marker gene.

The pre-publication data that we release via this website is embargoed for publication except for analyses of single chromosomes in single strains or single gene loci across multiple strains.

Similar results are observed in DE analyses of real data where the use of count sums instead of single - cell counts improves specificity and the ranking of relevant genes.

The present study shows that such a gradient is also seen at the level of single cell analysis, but reveals that there is considerable cell - to - cell variation within this gradient in the expression of pluripotency genes.

Genome - wide analysis of CNVs revealed that a single copy of the mdr1 gene on chromosome 5 and a novel amplification of the plasmepsin 2 and plasmepsin 3 genes on chromosome 14 also associate with raised piperaquine IC ₅₀ s.

The analysis of chromosomal inheritance patterns indicated a single functional and positional candidate gene and led to the discovery of the COLQ c. 1010T > C mutation; however, our approach does not exclude the possibility that another mutation exists in a novel CMS gene.