Sentences with phrase «analyzed by immunofluorescence»
After binding, cells were washed once (carefully using a 200 μl pipette to remove the medium) with RPMI with 0.5 % BSA solution and adherent cells were analyzed by immunofluorescence microscopy or bright field microscopy for quantification.
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Contribution of iPS cells to the gonad was analyzed by EGFP expression and immunofluorescence staining.