Thus,
the ancient horse genomes offer an unprecedented opportunity to investigate changes associated with domestication.
We therefore sequenced two
ancient horse genomes from Taymyr, Russia (at 7.4 - and 24.3-fold coverage), both predating the earliest archeological evidence of domestication.
They compared
these ancient horse genomes with already published genomes of 18 ancient and 28 modern horses.
Subsequent genetic studies have supported the NCC deficit hypothesis, most recently an April report in Science on the sequencing of 14
ancient horse genomes up to 4,100 years old; that's close to the estimated dawn of domesticated horses some 5,500 years ago.
Not exact matches
And since the cost of
genome sequencing has plummeted to one - thousandth of its initial cost, it's clear that the Neanderthal, a 700,000 - year - old
horse and the woolly mammoth will simply be the first of many
ancient genomes to be sequenced.
It is the latest in a series of breakthroughs in
ancient DNA, coming just months after the sequencing of the oldest - ever
genome, from a 700,000 - year - old
horse.
New techniques and very old bones overcome the limits of
genome sequencing for prehistoric
horses,
ancient cave bears, and even our own early ancestors.
But in 2013, ultramodern DNA extraction and sequencing techniques enabled researchers to access
ancient genetic codes and translate their evolutionary tales: Researchers in Denmark reconstructed a record - breaking 700,000 - year - old
horse genome, and geneticists in Germany began parsing the DNA of 400,000 - year - old hominids.
Whole
genome sequencing of modern and
ancient horses unveils the genes that have been selected by humans in the process of domestication through the latest 5,500 years, but also reveals the cost of this domestication.
This transformation left specific signatures in the
genomes of modern
horses, which the
ancient genomes helped reveal.
We therefore decided to sequence the
genome of
ancient horses that lived prior to domestication to directly assess how pre-domesticated
horses looked like genetically.»
This is something that was only detectable in the
horse in comparison to the
ancient genomes, as Przewalski's
horses were found to show a proportion of deleterious mutations similar to domesticated
horses.
This was apparent in the increasing levels of inbreeding found amongst domesticates, but also through an enhanced accumulation of deleterious mutations in their
genomes relative to the
ancient wild
horses.
Researchers have also delved into the
genomes of
ancient animals — the oldest so far is a 700,000 - year - old
horse.
This new work, which hints that other
horses may be represented in these
ancient genomes, shows «that [
horse] domestication could have been a process with many phases, experiments, failures, and successes,» says Ernest Bailey, a geneticist at the University of Kentucky's Gluck Equine Research Center in Lexington.
Genomes from
ancient horses show the genetic changes wrought by domestication — and their costs.
The sequenced
genome offers a glimpse at the
ancient horse and how equine genes have evolved.
Such tracks were also found among domesticated
horses but were almost absent from the
ancient genomes (Fig. 4A and SI Appendix, Figs.
The mitochondrial
genome sequences of the two
ancient horses clustered within the extensive diversity present in modern
horses, as previously reported (9, 12, 25).
This analysis revealed a statistically significant excess of shared derived polymorphisms between the
ancient and domesticated horses in the following quartets (Przewalski's, Domesticated; Ancient, Donkey), where «Domesticated» represents any of the six domesticated horse genomes included in this study (SI Appendix, section
ancient and domesticated
horses in the following quartets (Przewalski's, Domesticated;
Ancient, Donkey), where «Domesticated» represents any of the six domesticated horse genomes included in this study (SI Appendix, section
Ancient, Donkey), where «Domesticated» represents any of the six domesticated
horse genomes included in this study (SI Appendix, section S2.7).
These
ancient genomes reveal predomestic population structure and a significant fraction of genetic variation shared with the domestic breeds but absent from Przewalski's
horses.
In a recent study, researchers from the University of Copenhagen compared the complete
genomes of nine living and two
ancient Yakutian
horses from Far East Siberia with the
genomes of 27 modern domesticated
horses.