Animals were frozen at − 80 °C for 5 min before placing the tube into a thermal cycler and run
under the
following conditions: heat to 60 °C for 60 min
followed by inactivation of proteinase K by heating to 95 °C for 20 min.
Under the bill the
following animals were not required to be released on demand:  (1) animals «physically suffering irremediably» as long as a licensed veterinarian has issued a written, signed certification stating «that the prognosis for recovery is poor or grave even with comprehensive prompt and necessary veterinary care; «Â (2) dogs symptomatic for parvovirus and cats symptomatic for panleukopenia which
conditions have been confirmed by a licensed veterinarian in a written, signed certificate which also states «that the prognosis for recovery is poor or grave even with comprehensive prompt and necessary veterinary care;» (3)
animals which a veterinarian determined showed signs of rabies and (4) dogs declared «dangerous» by a court.   Â
• Proven record of effectively looking after
animals in shelters and facilities to ensure their physical and emotional wellbeing • Highly experienced in observing
animals» appearances and activities for general physical
conditions and obvious signs of illnesses • Well - versed in handling cleaning and disinfecting activities, to ward off potential diseases and ailments • Demonstrated expertise in restraining
animals for check - ups and medicine administration, while ensuring that they remain comfortable and safe • Proficient in determining the type and amount of food required for each
animal under care, and effectively creating correlating meal plans to meet their nutritional needs • Adept at washing, brushing and clipping
animals» coats to ensure that they remain clean and healthy • Well - versed in providing treatment through mouth and IVs and contacting vets to secure treatments • First - hand experience in up - keeping
animal pens and stables by ensuring that they are sanitizes on a regular basis • Focused on ensuring
animal safety during transport between facilities by
following set rules for
animal care and transport • Qualified to provide first aid to
animals in instances of emergencies to ensure their safety and wellbeing • Exceptionally talented in providing education to pet owners regarding the physical and emotional care of
animals