Sentences with phrase «anti-cd8 magnetic bead»

The magnetic bead - based immunoassay panel facilitates detection of more samples than any other available assay, allowing researchers to get the greatest possible amount of data from each run.

So the researchers turned to a machine made by Quanterix of Lexington, Massachusetts, that uses magnetic beads coated by an antibody that binds selectively and extremely tightly to the protein in the blood.

Magnetic beads have long been used to isolate nucleic acids as well as recombinant proteins.

The plasma containing magnetic beads then flows through the microchannels on the diagnostic chip in which the beads can be readily collected using a magnet to extract circulating exosomes from the plasma.»

«We used magnetic beads of 3 micrometers in diameter to pull down the exosomes in plasma samples,» Zeng said.

First, IgE antibodies from the patient's blood are isolated by interaction with magnetic beads that are coated with a different type of antibody.

What's new in KatharoSeq is that Minich integrated a sample pooling strategy, appropriate positive and negative controls, high - throughput DNA extraction and a magnetic bead - based DNA clean - up approach.

Each is composed of building blocks bound together, like a string of small magnetic beads — and just as magnets have poles, these building blocks have «plus» and «minus» ends.

Hiroshi Handa of the Tokyo Institute of Technology and his colleagues developed tiny magnetic beads — just 200 nanometers in diameter — that can be attached to drugs and other compounds.

Given these different methylation patterns, the researchers used specialized magnetic beads that hybridize with methyl - CpG - rich DNA regions in an attempt to separate nuclear DNA from organellar DNA in total genomic DNA samples.

Applying a magnet pulls out the magnetic beads, along with any linked gold beads and their associated bar - code DNA.

If the sequence of interest is in the sample, it forms a link between the gold beads and the magnetic beads.

They added these beads to the sample to be tested, and then added that mixture to a test tube containing magnetic beads coated with DNA strands that bind the other half of the DNA.

The kit adopts a novel purification method, using magnetic beads and phosphatidylserine - binding protein to isolate exosomes and other EVs from cell - culture medium and body fluids by a normal microcentrifuge.

Despite the ability of R3A to utilize both CCR5 and CXCR4 to infect cell lines, in human CD4 + T cells stimulated with anti-CD3 / anti-CD28 coated magnetic beads, CCR5 is downregulated causing transient resistance to R5 HIV [47].

Five days post-stimulation, the magnetic beads were removed and washed twice in fresh media.

The cells were stimulated with anti-CD3 / anti-CD28 coated magnetic beads at a 3 ∶ 1 bead to cell ratio [34].

Five days post-stimulation the anti-CD3 / anti-CD28 coated magnetic beads were removed from each of the three cultures (non-transduced (NTD), AdX4 - ZFNs, and AdR5 - ZFNs) and 2.5 million cells were seeded in each of four cultures that were subsequently infected with either Bk132 (primary X4 isolate), HxB2 (lab - adapted X4 isolate), R3A (R5X4 primary isolate), or media only (mock).

The putative DN cells were isolated by negative selection using anti-CD8 magnetic bead isolation followed by in vitro treatment with anti-CD8 antibody and complement.

Using a proprietary protocol DNA fragments are bound directly onto the surface of the magnetic beads, leaving unincorporated nucleotides, primers, primer dimers, and other contaminants in solution.

In a single reaction volume, Luminex has the ability to perform many different tests and use either magnetic beads or plain polystyrene beads.

Spleen cells were enriched for CD8 + and CD8 − T cells using magnetic beads.

Target bacteria were captured using antibody - coated magnetic beads and then encapsulated in picoliter - sized droplets with fluorescently labeled antibodies for detection.

After washing with PBS / 5 % FCS, stained cells were depleted by incubating the cell suspension with 500 μl Dynabeads magnetic beads (Life technologies, # 1141D) for 30 min at 4 °C on a rotator.

He still likes to tinker; only now he uses magnetic beads, atomic - force microscopes, and laser «tweezers» to explore the inner workings of the cell and the physical forces behind DNA replication.

This is a highly parallel sequencing approach that involves synthesizing short regions of identical DNA fragments on magnetic beads, packing millions of them into a chamber and then extending each of those molecules while detecting the addition of fluorescently labeled DNA building blocks or nucleotides.

For further information on MagSi - DNA cleanFIX and the fully range of magnetic beads available from AMSBIO please visit www.amsbio.com/DNA-sequencing-magnetic-purification.aspx or contact AMSBIO now on [phone] / [phone] or email [email protected].

For sorting human ILCs, tonsillar lymphocytes were depleted of B cells using CD19 magnetic beads (eBioscience).

With MagSi - Direct, you can start with a molecule of interest, attach magnetic beads to it and then use a magnet to isolate that molecule from any desired reaction mixture, together with any other molecules, complexes or even intact cells to which the starting molecule has bound.

We have a limited amount of product available for trial by users tired of the limitations of magnetic beads or who want to manipulate cells in a new way.

They injected a tiny magnetic bead into the nucleus and turned on a magnet to add a known force.

Cellular biology: Isolation and culture of various cell types; cryopreservation; Mixed Lymphocyte Reaction (MLR); cloning by limiting dilution; cytotoxicity assays using Chromium - 51 release; proliferation assay (CFSE and 3H - thymidine); ELISA; Elispot; magnetic beads separation.

Using the new solution, DNA fragments are bound directly onto the surface of the magnetic beads, leaving unincorporated nucleotides, primers, primer dimers, and other contaminants in solution.

HIF -1-alpha was immunoprecipitated using 0.5 mg HeLa Nuclear DFO treated whole cell extract (ab180880), 5µg of Rabbit polyclonal to HIF1 alpha and 50µl of protein G magnetic beads (+).

Following lysis and solid - phase capturing of mRNAs with streptavidin magnetic beads, samples were reverse transcribed using random primers.

HIF - 1 alpha was immunoprecipitated using 0.5 mg HeLa Nuclear DFO treated whole cell extract (ab180880), 5 µg of Mouse monoclonal to HIF - 1 alpha and 50 µl of protein G magnetic beads (+).

Based on 6.5 µm magnetic beads, it is compatible with both vacuum and magnetic wash stations.

Laboratory: Experience with laboratory applications in parasitology, immunology and molecular biology including, microscopy, BCA Protein Assay, ELISA, Flow Cytometry, Real Time qPCR, Luminex, Gas chromatography (GC) mass spectrometry, cotinine assays (saliva), magnetic bead cell separation (MACS, Percoll and Ficoll) and whole blood cultures.

Not exact matches

Helpful arrows directtheir movements, and a magnetic stylus pulls the metal beads up tocreate lines.

Plan Toys Bird Percussion / / Skip Hop Roll Around Hedgehog Rattle / / Hape Beaded Rain Maker / / Tegu Magnetic Building Blocks / / Squigz silicone suction cups / / Plan Toys Tie - Up Shoe / / Magnatab Upper Case Letters / / Hape Workbench

With a variety of sounds, moving beads, a magnetic hand, crinkly ears, a peek - a-boo head, and an assortment of colours and textures, the fun and education will never end when you present your baby with this interactive Christmas gift.

The beads in the video, made by Steve Mould, who hosts several BBC science shows, are not magnetic, either.

A team led by the Hutch's Marie Bleakley and by Warren Shlomchik of the University of Pittsburgh School of Medicine in Pennsylvania removes naïve T cells from grafts with a magnetic system that uses monoclonal antibodies bound to iron beads.

Evaluation of magnetic cellulose bead - based DNA extraction from faecal materials for high - throughput bacterial community analyses — Tanzila Afrin — Applied Entomology and Zoology

Following the provided protocol, a portion of the RNA - containing lysate (5 — 10 µl) was hybridized at 54 degrees C overnight to RNA specific magnetic capture beads in the presence of blocking buffers, proteinase K and preordered mRNA probe sets specific for the genes of interest: HMOX1, Nrf2, Keap1, Bach1, and GAPDH.

With the aid of a magnetic plate holder, capture beads containing the hybridized mRNA were washed and incubated with provided labeling probes.

High quality coating of magnetic silica beads with Steptavidin, Protein A, Protein G or other ligand specific molecules allows isolation of specific target molecules or cells out of large volumes or complex mixtures.

Computed tomographic scan in 1 infant and magnetic resonance imaging in another infant with prenatal Zika exposure show scattered punctate calcifications (A, B, C, and E; white arrowheads), very low forehead and small cranial vault (D), striking volume loss shown by enlarged extra-axial space and ventriculomegaly (all images), poor gyral development with few and shallow sulci (A and E; long white arrows), poor gyral development with irregular «beaded» cortex most consistent with polymicrogyria (F, white arrowheads), flattened pons and small cerebellum (D; black arrowhead and asterisk).

Using the dyes with magnetic nanoparticles and tiny fluorescent beads, allowed them to use the slime mold network as a biological «lab - on - a-chip» device.

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