Sentences with phrase «antibodies used in this study»

The antibody used in the study, called BG4, binds with quadruple helices and glows red.

Unlike other human antibodies under investigation that recognize both Zika and the closely related dengue virus, the antibodies used in this study exclusively target Zika, demonstrating a high specificity that could be important in avoiding potential side effects — such as enhanced dengue infection in regions where both viruses are endemic.

Antibodies used in these studies included: CD11b - phycoerythrin (CD11b - PE)(2 μg / ml), CD45.1 - PE (5 μg / ml), CD45.2 - PE (5 μg / ml; eBioscience, San Diego, California, USA), and F4 / 80 - APC (5 μg / ml; Caltag Laboratories Inc., Burlingame, California, USA).

Information for antibodies used in this study is provided in supporting information, Table S2.

The antibodies used in this study are listed in Table 1.

The antibodies used in this study includes COX - 2 (Cayman Chemicals, Ann Arbor, MI, USA), STAT6 for Western blot (Cell Signaling Technology, Danvers, MA, USA), for immunofluorescence (IF)(BD Pharmingen, San Diego, CA, USA), for ChIP (Santa Cruz Biotechnology, Santa Cruz, CA, USA), phospho - STAT6 (Cell Signaling Technology), p300 for Western blot, (Upstate, Lake Placid, NY, USA), for ChIP (Santa Cruz Biotechnology), normal rabbit IgG (Santa Cruz Biotechnology), mouse immunoglobulin (Ig) G1, κ Ig (BD Pharmingen), glyceraldehyde -3-phosphate dehydrogenase (Advanced ImmunoChemical, Long Beach, CA, USA), Cy3 - conjugated AffiniPure Goat anti-mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA) and PARP (Cell Signaling Technology).

Not exact matches

Such highly sensitive in vitro techniques permit the use of picomole quantities of an antigen in partially purified preparations for the isolation of monoclonal antibodies, which can in turn be used in biological studies and in immunochemical procedures for large - scale purification and isolation of that antigen.

In a study published in the May 29, 2014, edition of Molecular Ecology Resources, Duke researchers Peter Larsen, Ryan Campbell and Anne Yoder used high - throughput sequencing on sifaka blood samples to generate sequence data for more than 150,000 different sifaka antibodies — protective molecules that latch on to bacteria, viruses and other foreign invaders in the body and fight them off before they cause infectioIn a study published in the May 29, 2014, edition of Molecular Ecology Resources, Duke researchers Peter Larsen, Ryan Campbell and Anne Yoder used high - throughput sequencing on sifaka blood samples to generate sequence data for more than 150,000 different sifaka antibodies — protective molecules that latch on to bacteria, viruses and other foreign invaders in the body and fight them off before they cause infectioin the May 29, 2014, edition of Molecular Ecology Resources, Duke researchers Peter Larsen, Ryan Campbell and Anne Yoder used high - throughput sequencing on sifaka blood samples to generate sequence data for more than 150,000 different sifaka antibodies — protective molecules that latch on to bacteria, viruses and other foreign invaders in the body and fight them off before they cause infectioin the body and fight them off before they cause infection.

Masdeu plans to use study results to develop more sensitive tests to aid in the detection of attacking antibodies.

In a study recently published in the journal Nature Biotechnology, HSCI researchers at Harvard University and Massachusetts General Hospital (MGH), in collaboration with Boston Children's Hospital and Dana Farber Cancer Institute, have developed a non-toxic transplantation procedure using antibodies to specifically target blood stem cells in mice, an approach they hope will make blood stem cell transplants for these patients far less toxiIn a study recently published in the journal Nature Biotechnology, HSCI researchers at Harvard University and Massachusetts General Hospital (MGH), in collaboration with Boston Children's Hospital and Dana Farber Cancer Institute, have developed a non-toxic transplantation procedure using antibodies to specifically target blood stem cells in mice, an approach they hope will make blood stem cell transplants for these patients far less toxiin the journal Nature Biotechnology, HSCI researchers at Harvard University and Massachusetts General Hospital (MGH), in collaboration with Boston Children's Hospital and Dana Farber Cancer Institute, have developed a non-toxic transplantation procedure using antibodies to specifically target blood stem cells in mice, an approach they hope will make blood stem cell transplants for these patients far less toxiin collaboration with Boston Children's Hospital and Dana Farber Cancer Institute, have developed a non-toxic transplantation procedure using antibodies to specifically target blood stem cells in mice, an approach they hope will make blood stem cell transplants for these patients far less toxiin mice, an approach they hope will make blood stem cell transplants for these patients far less toxic.

Using the findings from this study, the team has designed vaccine immunogens to selectively trigger the cooperating antibody - producing B cells to cooperate to make broadly neutralizing antibodies in a manner that mimics broadly neutralizing antibody development in natural HIV infection.

The researchers now want to conduct follow - up studies to optimise the use of IgEnio — currently patients have to have IgE antibodies removed from their blood for a morning approximately once a week during the pollen season — and to test its use for treating inflammatory skin diseases, in which IgE antibodies are also implicated.

Using multiple statistical approaches to study a long - term pediatric cohort in Nicaragua, we show that risk of severe dengue disease is highest within a narrow range of preexisting anti-DENV antibody titers.

By examining the structure presented in this study, her team will work to determine how the antibodies could be used to kill the virus.

In the Nature Biotechnology study, the researchers used it to anchor proteins, and they also showed that the technique works on tissue that has been previously labeled with either fluorescent antibodies or proteins such as green fluorescent protein (GFP).

Ollmann Saphire says the next step is to study how known mutations in Marburg evade such antibodies and to use that information to devise second - line treatments.

In the study, researchers used the blood of seven people who survived Ebola Bundibugyo virus infection during the 2007 outbreak in Uganda to isolate a large number of B cells that produce antibodies, which are the small protein molecules capable of inactivating the viruIn the study, researchers used the blood of seven people who survived Ebola Bundibugyo virus infection during the 2007 outbreak in Uganda to isolate a large number of B cells that produce antibodies, which are the small protein molecules capable of inactivating the viruin Uganda to isolate a large number of B cells that produce antibodies, which are the small protein molecules capable of inactivating the virus.

But in those studies, interferon — delivered by an adenovirus — was used in combination with a cocktail of monoclonal antibodies.

Other researchers involved in the study, conducted entirely at NYU Langone, were Mariela Mitre, BE; James D'amour, BSc; and Moses Chao, PhD, whose laboratory developed the oxytocin receptor antibody used to track hormone activity.

In the current study, Vanderbilt researchers led by graduate student Andrew Flyak, the paper's first author, used a high - efficiency method they developed to quickly isolate and generate large quantities of monoclonal human antibodies from the blood of survivors of a 2007 outbreak in Uganda who were infected by the Bundibugyo ebolaviruIn the current study, Vanderbilt researchers led by graduate student Andrew Flyak, the paper's first author, used a high - efficiency method they developed to quickly isolate and generate large quantities of monoclonal human antibodies from the blood of survivors of a 2007 outbreak in Uganda who were infected by the Bundibugyo ebolaviruin Uganda who were infected by the Bundibugyo ebolavirus.

But neither team has yet shown that their bispecific antibodies can actually reduce reservoirs of HIV in monkeys, which are commonly used to study the AIDS virus.

«What we have shown in the Science study is that we now have the means to obtain real - time images of processes happening on the surface of intact HIV particles, which we now plan to use to screen the impact of drugs and antibodies that can shut it down,» he says.

To investigate why checkpoint inhibitors so often stop working, Velculescu; Valsamo Anagnostou, M.D., Ph.D., instructor of oncology at the Johns Hopkins University School of Medicine; Kellie N. Smith, Ph.D., a cancer immunology research associate at the Johns Hopkins University School of Medicine; and their colleagues at the Bloomberg ~ Kimmel Institute for Cancer Immunotherapy studied tumors of four patients with non-small cell lung cancer and one patient with head and neck cancer who developed resistance to two different checkpoint inhibitors: a drug called nivolumab that uses an antibody called anti-PD-1, or nivolumab used alone or in combination with a second drug called ipilimumab, which uses an antibody called anti-CTLA4.

Just as cells of the immune system use antibodies to recognize pathogens, researchers in this study designed antibodies to recognize a protein over-expressed by these cancer cells, namely the protein mesothelin.

They ordered the antibodies that had been used in more than 80 studies to measure irisin levels, and they analyzed them using Western blots.

By studying the connections between skin cells using AFM and other techniques that probe cells at the nanoscale, Sinha and his colleagues report that pathogenic antibodies change structural and functional properties of skin cells in distinct ways.

Two recent studies pointed to possible flaws in the methods used to identify irisin, with commercially available antibodies.

The study, appearing March 9 in the journal Scientific Reports, directly tested the antibodies used in previous analyses and showed that they cross-reacted with proteins other than irisin, yielding a false positive result.

In the current study, the researchers used high - affinity antibodies to «label» the cannabinoid receptors so they could be seen using various microscopy techniques, including electron microscopy, which allowed very detailed visualization at individual synapses, or gaps between nerve cells.

«Currently, there is a lot of focus on the use of antibodies transferred passively or through a vaccine to prevent infection in infants, however this study cautions against that and suggests that broadly neutralizing antibodies may actually aid in enhancing transmission from mother to child,» added Sagar, an attending physician in infectious diseases at Boston Medical Center.

In the new study, CDC's Nicholas Komar and Kasen Riemersma — who's now at the University of California, Davis — used existing blood samples from white - tailed deer, raccoons, moose, and coyotes collected between 2009 and 2014 in 19 states, both in the center of the lone star tick's range and its periphery, and tested them for antibodies against the Heartland viruIn the new study, CDC's Nicholas Komar and Kasen Riemersma — who's now at the University of California, Davis — used existing blood samples from white - tailed deer, raccoons, moose, and coyotes collected between 2009 and 2014 in 19 states, both in the center of the lone star tick's range and its periphery, and tested them for antibodies against the Heartland viruin 19 states, both in the center of the lone star tick's range and its periphery, and tested them for antibodies against the Heartland viruin the center of the lone star tick's range and its periphery, and tested them for antibodies against the Heartland virus.

In this study, patients began treatment with chemotherapy using cyclophosphamide and topotecan and the experimental monoclonal antibody hu14.18 K322A.

Pembrolizumab, an antibody drug already used to treat other forms of cancer, can be effective in the treatment of the most common form of mesothelioma, according to a new study led by investigators from the Perelman School of Medicine at the University of Pennsylvania.

In the new study, researchers used specially designed antibodies in the mice to first wipe out rogue beta - cell - killing T cells from the immune systeIn the new study, researchers used specially designed antibodies in the mice to first wipe out rogue beta - cell - killing T cells from the immune systein the mice to first wipe out rogue beta - cell - killing T cells from the immune system.

While viral escape has not been observed in the NHP models of immunoprophylaxis described above, virus can evolve in response to neutralizing antibodies in HIV - positive patients [16], [17] and early studies showed escape of patient virus from passive antibody - mediated protection when a single mAb was used [18].

The present findings are derived from studies using CD4KO mice and thus, raise the question as to whether the CD4 + T cell - independent immune mechanisms in CD4 KO mice differ from those involved in corneal allograft rejection in wild - type mice whose CD4 + T cells population have been depleted with monoclonal antibodies.

And researchers understand use of PrEP and other HIV prevention strategies may limit their ability to study the natural evolution of antibody responses in HIV - infected individuals.

For example, their studies showed that avian FcRY, unlike mammalian FcRn, uses multiple domains to bind to its antibody, changing its conformation in the process.

BUFFALO, N.Y., Feb. 15, 2017 / PRNewswire / — Today, a research team from Everon Biosciences, Inc. and Roswell Park Cancer Institute has published a new study in Proceedings of the National Academy of Sciences of the United States of America (PNAS) describing a new biomarker of senescent cells and a specific monoclonal antibody that could potentially be used to develop -LSB-...]

Major CFS symptom relief during cancer chemotherapy in a patient with synchronous CFS and lymphoma spurred a pilot study of B - lymphocyte depletion using the anti-CD20 antibody Rituximab, which demonstrated significant clinical response in three CFS patients.

During the 1980s, Marder led studies using antibodies and the technique of immunocytochemistry to reveal the presence of many different neuromodulators in nerve fibres around the STG of crabs, including serotonin, proctolin and GABA.

Made major contributions to the field of immunology through his studies on immune tolerance in animal models, with particular focus on transplantation tolerance and autoimmunity, in addition to pioneering the use of therapeutic monoclonal antibodies against T cells, resulting in the development of Campath - 1H, the first humanised monoclonal antibody to be used therapeutically.

Vaccines are used to offer protection against a number of diseases and so ensuring their effectiveness is important, and a study published in the journal Vaccine has reported that flu vaccines given in the morning brought about a greater immune (measured by antibody levels) response than those given in the afternoon.

In other applications, they are used to deliver genes that provide instructions for generating molecules such as antibodies or fluorescent proteins that help researchers study, identify, and track certain cells.

In a paper published online Aug. 31, by Nature Medicine, researchers report that using a monoclonal antibody called anti-CD47, which blocks the «don't - eat - me» signal on malignant cells, to treat mice with an intact immune system provides a much more lifelike way to study and develop an immune - based cancer therapy.

These whole proteome arrays, available in 100 copies each, will be used to study autoimmunity in patient samples and to validate the specificity of antibodies.

SSI offers vaccination studies in mice including 1 - 2 control groups and full immunogenicity analysis including CMI and humoral immunogenicity using Flow cytometry (B and T cells) and ELISA (cytokines and antibodies).

IAVI launched the Neutralizing Antibody Consortium (NAC) in 2002 to study the molecular structure and biochemistry of known broadly neutralizing antibodies and to use this information to design (reverse engineer) potentially powerful AIDS vaccine candidates.

Post says the study is a new take on monoclonal antibody drugs that have been used in humans.

A study performed by vaccine manufacturer Vanguard using a COMBINATION vaccine (one which contains several different vaccines) tested the efficiency of the vaccine in stimulating antibodies in an immune response.