PBS was used instead of the primary
antibody as the negative control, and is shown in the inset.
Not exact matches
EdiGene Knockout Cell Lysates can be used to validate
antibodies for specificity or used
as high - quality
negative controls for Western blot analysis.
An isotype
control for the secondary
antibody was used
as a
negative control in both samples.
A sample without a primary
antibody can be used
as a
negative control to distinguish specific and non-specific signal from the secondary
antibody.
While we could not use the XT slides with secondary
antibody only
as a
negative control (due to the limited material), we did stain smears of ticks fed on clean mice using all
antibodies (primary and secondary).
Negative controls including the secondary
antibody alone and uninfected monkey tissue were used,
as was an ex vivo section of infected macaque brain tissue [43] for the positive
control.