Sentences with phrase «antibody loading control»
Not exact matches
Specifically, 1 unit of access comprising a vaccination / challenge study with 3 groups of 6 ferrets (control and 2 vaccine groups), to include vaccination / boost, intra-nasal challenge with influenza virus, monitoring of virus load (nasal washes), disease progression and immune responses (antibody, IFN gamma ELISA and ELISpot).
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Some caution is required when using this antibody as a loading control as COXIV expression can vary under some manipulations.
Immunohistochemistry (Formalin / PFA - fixed paraffin - embedded sections)- Anti-alpha Tubulin antibody [DM1A]- Loading Control (ab7291)
However, be aware that many proteins run at the same 16kD size as COX IV - our VDAC1 / Porin antibody makes a good alternative mitochondrial loading control for proteins of this size.
An alternative mitochondrial loading control is Mouse monoclonal to COX IV antibody [20E8](ab14744).
Immunocytochemistry / Immunofluorescence - Anti-alpha Tubulin antibody [DM1A]- Loading Control (ab7291)
Excellent as a protein loading control antibody.
Immunohistochemistry (Frozen sections)- Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)
The cells were then incubated with the antibody ab16056 at 1µg / ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4 °C.
The unit of access comprises a vaccination / challenge study with 3 groups of 6 ferrets (control and 2 vaccine groups), to include intra-nasal challenge with influenza virus, monitoring of virus load (nasal washes), disease progression and immune responses (antibody, IFN gamma ELISA and ELISpot).