Sentences with phrase «antigen retrieval»

The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was blocked and incubated with the antibody for 1 hour.

A heat mediated antigen retrieval step was performed with Tris - EDTA buffer (pH9).

Slides were incubated in antigen retrieval buffer (DAKO, Carpinteria, CA,) supplemented with 0.3 % Tween 20 and boiled at 100 ˚C for 10 minutes.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Requires heat - induced antigen retrieval where aldehydes are used as fixatives.

Tissue sections were placed in 10 mmol / L citric acid buffer (pH 6.0) and subjected to antigen retrieval by microwaving for 20 minutes after deparaffinization and rehydration.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins.

Sections were PFA fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in 10 % serum for 10 minutes at 25 °C.

Tissue was fixed with paraformaldehyde and blocked with 5 % serum for 1 hour at 21 °C; antigen retrieval was by heat mediation in a citrate buffer.

Tissue was fixed with 10 % buffered formalin and blocked with 5 % serum for 60 minutes at 21 °C; antigen retrieval was by heat mediation in a 10mM sodium citrate buffer pH6.

Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.5 % Triton X-100 and blocked with 10 % serum for 1 hour at 25 °C; antigen retrieval was by heat mediation in 10 mM citrate buffer, pH 6, for 20 minutes at 97 °C in a water bath.

Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using 10 mM citrate buffer pH 6.0.

Tissue was fixed and paraffin - embedded, antigen retrieval was by heat mediation in Tris / EDTA buffer pH9.