Immunodiagnosis involves looking for
antigens of microfilaria or antibodies against the microfilaria using monoclonal antibody techniques.
Not exact matches
For example, an infected dog within the household may be the source
of heartworm and therefore should be tested for heartworm
antigen and
microfilariae.
There is generally no need to test a dog for
antigen or
microfilariae prior to ~ 7 mo
of age.
Performance
of a
microfilariae test is recommended at the time the
antigen test is performed (6 mo after the adulticide treatment).
Rather than visually searching for the
microfilaria, blood screening
antigen tests can detect the presence
of adult female worms even before they've had babies.
And, finally, in any dog with a very low heartworm burden — say, just a couple
of worms — the
microfilariae may not be detected (this can also cause a false negative on the
antigen test).
Up to 1 %
of infected dogs are
microfilaria - positive and
antigen - negative.
The preventative won't affect
antigen test results and it can prevent the spread
of heartworm in the shelter and prevent infection in an animal, even if
microfilaria (heartworm larvae) are already present in the bloodstream.
One 13 - years - old mixed male, with high number
of microfilariae and
antigens - positive, died during the therapy.
As a matter
of fact, none
of seven dogs from a non-endemic area in Central Italy (Fermo, Marche), with D. immitis
microfilariae (fig. 1,2,4) in the blood and patent disease, proved seropositive with two
antigen tests (PetChek and Wittness).
Diagnosis
of canine dirofilariosis in animals which originate from a low - endemic region is difficult for 3 main reasons: 1) possible absence
of circulating
microfilariae (occult disease), 2) high frequency
of a low number
of adults, which in turn produces false negative results, using either ELISA or latex agglutination test, 3) possible false sero - positive results, due to cross-reaction with adult
antigens of Dirofilaria (Nochtiella) repens (Schrey, 1996), the agent
of subcutaneous dirofilariasis causing chronic pruritic dermatitis in dogs (Tarello, 1999).
The reliability
of heartworm testing, which depends on the presence
of heartworm
antigen or
microfilariae, is affected by the relative lack
of both in cats.
However, in stage one the disease has not yet progressed to the point where the heartworms will have produced a new generation
of microfilariae and dog's body will not yet have produced
antigens in an amount sufficient for detection.
If the
antigen screening test is positive, another blood test is available to identify the concentration
of microfilariae in circulation.