Sentences with word «aprotinin»
Briefly, retinal samples were homogenized at 4 °C in buffer containing 10 mM Tris (pH 7.4), 1 mM EDTA, 0.15 M sodium chloride, 0.5 % NP - 40, with protease inhibitors (20 µg / ml aprotinin, 5 µg / ml leupeptin, and 1 mM phenylmethane sulfonyl fluoride - PMSF).
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Cells were lysed with TN1 lysis buffer (50 mM Tris, 125 mM NaCl, 1 % Triton X-100, 10 mM EDTA, 10 mM sodium fluoride and 10 mM sodium pyrophosphate) supplemented with protease inhibitor cocktail (1.2 mM AEBSF, 0.46 μM aprotinin, 14 μM bestatin, 12.3 μM E-64, 112 μM leupeptin, 1.16 μM pepstatin)(Amresco; Solon, OH)-RRB- and 1 mM sodium orthovanadate (Enzo Life Sciences; Farmingdale, NY).
Kemppainen RJ, Clark TP, Peterson ME: Aprotinin preserves immunoreactive adrenocorticotropin in canine plasma.
Kemppainen RJ, Clark TP, Peterson ME: Preservation effect of aprotinin on canine plasma immunoreactive adrenocorticotropin concentration.
The nuclear pellet was resuspended in a second lysis buffer containing 50 mM HEPES (pH 7.9), 0.4 M sodium chloride, and 1 mM EDTA, with protease inhibitors (20 µg / ml aprotinin, 5 µg / ml leupeptin, and 1 mM PMSF).
Hippocampal and entorhinal cortex tissue samples were homogenized in 10 volumes of RIPA lysis buffer (50 mM Tris, pH 7.5, 150 mM NaCl, 0.1 % sodium dodecyl sulfate and 0.5 % deoxycholate, and 1 % NP40) containing a cocktail of protease and phosphatase inhibitors (20 mg / ml each of pepstatin A, aprotinin, phosphoramidon, and leupeptin; 0.5 mM 4 -(2 - aminoethyl) benzenesulfonyl fluoride hydrochloride; 1 mM EGTA; 5 mM fenvalerate; and 5 mM cantharidin).
For this protocol, samples were lysed in 1 × RIPK2 lysis buffer [50 mM Tris (pH 7.5), 0 mM MgCl2, 1 % Triton X-100, 1 mM dithiothreitol, 1 mM EDTA, 1 mM EGTA, with freshly added 1 mM β - glycerophosphate and protease inhibitors (phenylmethylsulfonyl fluoride and aprotinin)-RSB- and immunoprecipitated overnight using 1.5 μg rabbit anti-RIPK2 antibody.
iPS - RPE cells were collected on ice in lysis buffer (10 mM HEPES, 1 % Triton, 150 mM KCl, 1 mM PMSF, 10 ng / ml leupeptin, 1 mM DTT, 50 ng / ml aprotinin, 10 mM NaF, and 100 µM sodium vanadate) and incubated for 30 min on a tube rotator at 4 °C.