Recent developments in the field of metagenomics and single - cell genomics, however, have allowed the reconstruction
of archaeal genomes directly from environmental samples without prior cultivation.
Although environmental surveys had started to reveal a plethora of uncultured
archaeal lineages during the 1990s, in - depth knowledge about their diversity, ecology, and evolution remained limited.
As a member of the consortium, I'm focusing my studies towards developing imaging - based tools to observe the cell cycle of the archaea H. salinarum as a proof - of - concept for live - cell imaging
in archaeal model organisms.
The genome of M. acetivorans C2A is by far the largest of all sequenced
archaeal genomes.
The putative
archaeal host existed in a stable symbiotic relationship with one or more bacterial species, with the capacity for both gene and lipid exchange between species.
«Well, I used to laugh at the idea,» says Mark Young, a Montana State University biologist who leads a research team that gathers new
archaeal viruses from superhot aquatic environments in Yellowstone National Park and other places around the world.
«The results of our genetic analysis (DNA - based quantitative PCR and next - generation sequencing), together with results obtained from infrared spectroscopy imaging, allowed us to link lower levels of sebum [the oily secretion of sebaceous glands] and thus reduced skin moisture with an increase of
archaeal signatures.»
Degradation shaped bacterial and
archaeal communities with predictable taxa and their association patterns in Zoige wetland at Tibet plateau — Yunfu Gu, Scientific Reports
Our aim is to shed light on the evolutionary path by which
archaeal proteins gave rise to the eukaryotic cytoskeleton.
Here we compared the methane production between runnel and polygonal ponds using stable isotope ratios, 14C signatures, and investigated potential methanogenic communities through high - throughput
sequencing archaeal 16S rRNA genes.
The lineages of these groups are not restricted to extreme habitats, as was once thought common
for archaeal species; rather, archaea are widespread and occur in all thinkable environments on Earth, where they can make up substantial portions of the microbial biomass.
Disa will work on the genomic exploration of «microbial dark matter», focusing on
new archaeal lineages and viruses, using both lab - and bioionformatics - based approaches.
Since then, our knowledge
on archaeal diversity has increased rapidly.
On the basis of our current understanding, much
archaeal diversity still defies genomic exploration.
Schematic phylogenetic tree including major
archaeal groups for which genomic data are currently available.
The researchers compared the sequence to that of other sequenced methanogens to identify a distinct set of genes characteristic of
archaeal methanogens.
Starting his first postdoc here in Ettema - lab his work will involve the exploration of poorly
described archaeal lineages and the development of novel methods to investigate syntrophic relationships in microbial communities.
Currently only 35 bacterial and
archaeal phyla are recognized on the basis of classical approaches to microbial taxonomy.
Spang et al. review the diversity of Archaea and their genomes, metabolomes, and history, which clarifies the biology and placement of recently
discovered archaeal lineages.
Recently, cultivation - independent sequencing methods have produced a wealth of genomic data for previously
unidentified archaeal lineages, several of which appear to represent newly revealed branches in the tree of life.
Ten years ago in the early days of genome sequencing, researchers scoured the genomes of 580 bacterial and
archaeal species for large genes.
The wealth of
novel archaeal genomic data represents a treasure trove for generating testable hypotheses on the metabolic and cellular features of these archaea and will thus help to unveil their thus far poorly characterized biology.
«Typically, every methane seep site hosts several different groups of
archaeal methane - oxidizers,» Hatzenpichler said.
The variations in
archaeal abundance among the age groups were statistically significant and unexpected.
This study sheds new light
onto archaeal genome evolution, the deep roots of archaea and the phylogenetic placement of DPANN.
My aim is to find
Archaeal cytoskeletal proteins and identify their structure using spinning disk confocal microscopy.
Comparisons of bacterial and
Archaeal Communities in the Rumen and a Dual - Flow Continuous Culture Fermentation System using Amplicon Sequencing — I J Salfer — Journal of Animal Science
He proposes that the nucleus might have come from a virus that once
infected archaeal cells but eventually began sticking around.
Knowledge of
archaeal biological diversity and their role in evolution has rapidly expanded in the past decade.
Efforts to obtain and study genomes and enrichment cultures of uncultivated microbial lineages will likely further expand our knowledge
about archaeal phylogenetic and metabolic diversity and their cell biology and ecological function.
As the closest
known archaeal relative of eukaryotes, Loki helps to answer that question.
A team adapted the polymerase chain reaction and other methods to spot their targets and isolated and
characterized archaeal DNA from dozens of biopsies of human skin, lungs, noses, and guts, including intestinal tissue from people with inflammatory bowel disease.
Orphan and her team plan to use the genomic information from the paired methane -
oxidizing archaeal and bacterial partners to develop deeper insights into the physiology and mechanisms of interaction and energetic exchange between different methanotrophic consortia coexisting in the environment.
For this study, Hatzenpichler and his colleagues used sediment samples collected from deep - sea methane seep sediments off the coasts of Oregon and California for a series of incubation experiments, tracking the slow growth of methane - metabolizing bacterial and
archaeal populations.
«From a practical standpoint, the ability to direct the specific, addressable destruction of DNA... could have considerable functional utility, especially if the system can function outside of its native bacterial or
archaeal context,» they wrote.
In collaboration with Peter Wolf of the Medical University of Graz, the team also
correlated archaeal abundance with skin dryness, as middle - aged persons have higher sebum levels and thus moister skin than the elderly.
Klare JP, Bordignon E, Engelhard M, Steinhoff HJ (2011) Transmembrane signal transduction in
archaeal phototaxis: The sensory rhodopsin II - transducer complex studied by electron paramagnetic resonance spectroscopy.
At present, STSV1 is the
largest archaeal virus that has been isolated and studied.
Tide as Steering Factor in
Structuring Archaeal and Bacterial Ammonia - Oxidizing Communities in Mangrove Forest Soils Dominated by Avicennia germinans and Rhizophora mangle — Magalí S. Marcos et al. — Microbial Ecology
I believe we can re-construct and characterise
complex Archaeal cytoskeleton networks by co-expressing multiple proteins using this genetically tractable organism.
Plant, root, and soil microbiome Compositional shifts in root - associated bacterial and
archaeal microbiota track the plant life cycle in field - grown rice.
I am currently using fission yeast as a system to express and
image Archaeal proteins.
Four labs simultaneously engineered the bacterial and
archaeal CRISPR - Cas9 system to induce precise cleavage at mammalian genomic loci1 — 4.
Insights into substrate specificity of geranylgeranyl reductases revealed by the structure of digeranylgeranylglycerophospholipid reductase, an essential enzyme in the biosynthesis of
archaeal membrane lipids.
Spo11 introduces DNA breaks in the chromosomes and is homologous to the DNA cleavage subunit of
archaeal topoisomerase IV.
Sogin and his co-workers have demonstrated the power of pyrosequencing by amplifying the V6 variable region of 16S rRNA from marine deep water and hydrothermal vent samples, analyzing over 900,000 bacterial and
archaeal tags from the vent system and revealing greater taxonomic richness (over 20,000 OTUs observed at 3 % sequence divergence) than has previously been reported for any microbial habitat [39,42,43].