Sentences with phrase «artificial chromosome»

Overlapping «cassettes» of 5 to 7 kilobases (kb), assembled from chemically synthesized oligonucleotides, were joined by in vitro recombination to produce intermediate assemblies of approximately 24 kb, 72 kb («1/8 genome»), and 144 kb («1/4 genome»), which were all cloned as bacterial artificial chromosomes in Escherichia coli.

Several other technical advances helped, including the development of huge yeast artificial chromosomes, so - called «megaYACs», which can store up to 1.4 million pairs of DNA in one big chunk — 35 times more than can be stored in bacteria, the conventional way to clone DNA.

He described ongoing work at The J. Craig Venter Institute to produce artificial chromosomes and achieve genome transplantation as stepping stones toward the propagation of synthetic genomes.

We have determined the nucleotide sequence of nearly all of the ∼ 120 - megabase euchromatic portion of theDrosophila genome using a whole - genome shotgun sequencing strategy supported by extensive clone - based sequence and a high - quality bacterial artificial chromosome physical map.

These improvements push the boundaries of nucleic - acid detection and fragment sizing, affecting applications such as single - cell nucleic acid analysis, PCR - free preparation of NGS library preparation, bacterial artificial chromosome clone sizing, and exosome analysis.

Earnshaw, along with colleagues from the National Institutes of Health and the University of Nagoya in Japan, have finally found a way around the problem and are now conducting the foundational research needed to build functional artificial chromosomes.

This included advances in recombinant DNA technology — for example, the creation of recombinant artificial chromosomes — combined with semi-automated (invented in the Leroy Hood lab in 1986) and later automated DNA sequencing.

Notably, these include the J. Craig Venter Institute's 2008 synthesis of the first artificial chromosome.

Mammalian artificial chromosomes are a promising new system for gene delivery.

Non-coding-regulatory regions of human brain genes delineated by bacterial artificial chromosome knock - in mice.

Szostak had started a bold project aimed to construct artificial chromosomes that would enable scientists to clone a large cluster of human genes — 10 to 20 — on a single linear molecule of DNA in yeast cells.

Waterston's solution was to exploit yeast artificial chromosomes, or YACS, which could handle much bigger pieces of DNA.

As part of the international effort to sequence the 17 — billion — base - pair hexaploid bread wheat genome (2n = 6x = 42 chromosomes), we constructed a bacterial artificial chromosome (BAC)-- based integrated physical map of the largest chromosome, 3B, that alone is 995 megabases.

He anticipates the development of specialized artificial chromosomes — a «good health» artificial chromosome, for instance — that could routinely be inserted into human embryos.

Scientists produced an artificial chromosome in the lab to investigate how cells renew themselves — a process known as cell division.

Our Post-Darwinian Future Pluripotentstem cells, gene targeting, and artificial chromosomes could leapfrog over evolution and let us take control of our genome, maybe even turn ourselves into a whole new species.

«Based on what we know, the artificial chromosome is going to be the best way to modify the genome,» says Lee Silver, a professor of molecular biology and public policy at Princeton University.

Venken first sought a vector that could handle large amounts of DNA efficiently, settling on a bacterial artificial chromosome, which was designed a couple years ago and is known to maintain only a few copies of the DNA (though it can produce a high number of copies if it is induced).

Some of the pieces also contained «watermarks»: a few extra or different bases here and there that distinguish an artificial chromosome from a natural one.

To gain access to the gene - containing portion of the barley genome at high resolution, Close and his team identified and sequenced 15,622 BACs or bacterial artificial chromosomes — small fragments of the barley DNA linked to other DNA to constitute a circular molecule that can replicate and be propagated inside an E. coli bacterial cell, enabling researchers to produce copies of each BAC for DNA sequencing one small piece of the barley genome at a time.

The first step was to insert an artificial chromosome containing the cumbersome human antibody gene into the nucleus of a cow cell.

The first significant insight into the genome came from the analysis of 10 bacterial artificial chromosome (BAC) sequences (Kovach et al. 2010).

BAC stands for bacterial artificial chromosome, referring to the way the HD gene was put into the mouse.

High - efficiency counterselection recombineering for site - directed mutagenesis in bacterial artificial chromosomes.

Here we describe the double - end sequencing and mapping of 84,507 bacterial artificial chromosomes (BACs) generated from AB2.2 ES cell DNA (129S7 / SvEvBrd - Hprtb - m 2).

Bird, A. W., Erler, A., Fu, J., Hériché, J. K., Maresca, M., Zhang, Y., Hyman, A. A. and Stewart, A. F. High efficiency counterselection recombineering for site - directed mutagenesis in bacterial artificial chromosomes.

The CCHa2 - R - GAL4:: p65 construct (Fig 2E) was generated with bacterial artificial chromosome (BAC) recombineering techniques [57] in P [acman] BAC clone CH321 - 87C13 [58](Children's Hospital Oakland Research Institute, Oakland, CA).

Methods will be developed for rapidly following de novo centromere assembly in mammalian cells after introduction of large arrays of centromeric alphoid DNA carried on bacterial or yeast artificial chromosomes.

McCombie says that the pace of the Arabidopsis sequencing project was accelerated by a first - of - its - kind effort to use high - throughput «whole - genome random BAC fingerprint analysis» to map a large eukaryotic genome in its entirety and provide an ordered set of DNA clones for sequencing (BAC, bacterial artificial chromosome).