Sentences with phrase «as a negative control in»

An isotype control for the secondary antibody was used as a negative control in both samples.

Not exact matches

«Divergence in external tariffs and internal rules as well as absence of common institutions and a shared legal system, necessitates checks and controls to uphold the integrity of the EU single market as well as of the U.K. market... This, unfortunately, will have negative economic consequences,» it said.

«The consortium of 40 + banks (known as R3cev) which aims to do just that will inevitably develop something which: is permissioned (for users and developers like the apple app store), privatized, has fees, will not be entirely transparent to everyone, will not be open - source, it will definitely be inflationary to accommodate monetary policy of debasement and fractional reserve schemes, it will facilitate negative interest rates, central control of accounts for suspension / freezing of funds, bail - ins, bail outs, capital controls and transactions will include the identity of both sender and receiver and store that information in a centralized location for the convenience of hackers.»

Sabet, who worked in the White House's Office of National Drug Control Policy during President Obama's first term and serves as director of the Drug Policy Institute at the University of Florida, said the negative consequences of marijuana legalization include advertising aimed at kids, an increase in drugged - driving incidents and a spillover of marijuana from Colorado into surrounding states, where the drug remains illegal.

Concerns over negative economic impacts, such as a widening trade deficit with China, increased control of certain sectors by Chinese state - owned enterprises (SOEs), and intensified competition in the labour market for mid-skilled and less educated Canadian workers in particular; [1]

For example, ISS views as negative a single trigger, such as automatic vesting upon a change in control.

In a richly valued market, that sort of risk control is most appropriately established using call options having a strike price situated at about the point where various trend - following measures would turn negative — what is known in finance as a «contingent position» because the position creates its own exit if the market deteriorates further without an interim recovery - and particularly if it deteriorates abruptlIn a richly valued market, that sort of risk control is most appropriately established using call options having a strike price situated at about the point where various trend - following measures would turn negative — what is known in finance as a «contingent position» because the position creates its own exit if the market deteriorates further without an interim recovery - and particularly if it deteriorates abruptlin finance as a «contingent position» because the position creates its own exit if the market deteriorates further without an interim recovery - and particularly if it deteriorates abruptly.

They are «dimly conscious» in two senses: (1) as experiences, they do not normally rise to the stature of conscious centers competing for control of the organism, but they have appetitions and aversions in their own right so that it seems appropriate to label them «dimly conscious»; (2) they are perceived only dimly by the members of the regnant society, i.e., the regnant society has these particular occasions as dim, vaguely felt, negative «scars» on the data of what is clearly perceived in full consciousness.

As a result of this all too typical pattern of unjust land distribution, for example, 77 percent of the land in El Salvador, where fourteen families control the wealth, is suffering from erosion, according to a recent study.25 A more just distribution of land would greatly reduce the negative impact on the environment.

There were her black - and - white negative thoughts, her rejection of help, her insistence on a definition of life as being able to take care of oneself, her use of objectified terms (such as the new life stage of «miserable existence» to replace merely «feeling miserable»), her unsolicited speaking for others, her legalistic analysis of the problem of euthanasia and doctor - assisted suicide, her exaggeration of minor and temporary discomforts, her refusal to accept family support — cumulatively resulting in her choice to be «in control» and die.

This feminist psychiatrist predicts «extremely negative psychological sequelae» should the «fundamental right to abortion as a legitimate option in attaining control over one's own bodily self be suddenly renounced.»

Women run 5 to 7 times the risk of death with cesarean section compared with vaginal birth.14, 29 Complications during and after the surgery include surgical injury to the bladder, uterus and blood vessels (2 per 100), 30 hemorrhage (1 to 6 women per 100 require a blood transfusion), 30 anesthesia accidents, blood clots in the legs (6 to 20 per 1000), 30 pulmonary embolism (1 to 2 per 1000), 30 paralyzed bowel (10 to 20 per 100 mild cases, 1 in 100 severe), 30 and infection (up to 50 times morecommon).1 One in ten women report difficulties with normal activities two months after the birth, 23 and one in four report pain at the incision site as a major problem.9 One in fourteen still report incisional pain six months or more after delivery.9 Twice as many women require rehospitalization as women having normal vaginal birth.18 Especially with unplanned cesarean section, women are more likely to experience negative emotions, including lower self - esteem, a sense of failure, loss of control, and disappointment.

The overwhelming focus on negative aspects of life and conflict in the news colors our perception as well and can either numb or desensitize us or increase our general anxiety load, or leave us feeling helpless over events outside our control.

As a built in feature of the Hazelbaker FingerFeeder, this infant control can be augmented by a gentle squeeze of the milk container should the baby not be able to generate adequate negative pressure to draw milk from the device.

In closing their article, Price et al. stated that organizations, such as the Australian Breastfeeding Association and the Australian Infant Mental Health Association, were unduly negative towards controlled - crying techniques and based their positions on research that had not been «updated since the mid-2000s.»

The NRA, infuriated over Cuomo's Safe Act, which is billed as the toughest gun - control legislation in the nation, is preparing to give the Cuomo / Hochul ticket a negative rating while backing Astorino.

With Kurantowicz as director, Voters for Good Government made a splash in 2012 by spending about $ 300,000 on a last - minute burst of negative ads and mailings aimed at trying to defeat a half - dozen Democrats and flip control of the state Senate.

Asked if Democrats» winning control of Congress in next year's elections would benefit rather than hurt the country, more see it as a positive than negative — but opinions are not resolute.

«The most cost - effective measure we could take is the control of feral pigs at a cost of $ 2 million per annum in targeted location across the Basin, as they have a negative impact on both native plants and animals,» she said.

Participants in a control group practiced a different technique known as cognitive reappraisal, in which they learned to generate fewer negative thoughts.

PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Used as the parental line in deriving B - THP - 1 / DC - SIGN cells (5013) Negative control line used in DC - SIGN

Untreated cells (negative control) were grown in parallel for the same amount of time as treated cells.

As a negative control, unedited parental cells were grown in parallel for the same amount of time.

(A) GUS activity with and without 10 µm of the TNT ligand in paired leaves from transgenic plants serving as negative controls and transgenic plants containing the complete signal transduction system.

In all negative tumors, occasional positive tumor cells or coexisting reactive lymphocytes were present and served as internal positive controls (arrows in C, rightIn all negative tumors, occasional positive tumor cells or coexisting reactive lymphocytes were present and served as internal positive controls (arrows in C, rightin C, right).

[14, 15] As a negative control, a second pair of TMA slides was incubated overnight in ACE block only.

Posterior probability distributions for the difference in DNA abundance between air swab negative controls and placenta (blue), saliva (red), vaginal swabs (orange), and other negative controls (green) as estimated by quantitative PCR analysis of 16S rRNA gene abundance for PSP (top) and MO BIO extractions (bottom).

PCR contamination occurring during nested PCR was evaluated by including at least one third as many water controls as test samples in each PCR experiment and were always negative.

ChIP - Seq for DAXX was successful, as the strength of the signal in the experimental sample (Figure 1A, top panel) was high compared to the signal from the negative control, the input (Figure 1A, bottom panel).

A) Negative of photographs (x20) of aortic rings (lateral view) after 10 days of incubation in a 3D collagen gel overlayed with complete medium in the presence of 20 mg / mL VEGF, 0.05 % DMSO (the vehicle taken as a control), or kahweol at 1, 5 and 25 µM (K1, K5, K25, respectively).

DU145 cells (XMRV - uninfected)[39], and 22Rv1 cells carrying at least 10 integrated copies of XMRV [31], were used as a negative and positive control, respectively, in XMRV FISH assays.

In all our immunohistochemical analyses of PRLR in mouse mammary tissue, we used PRLR - deficient mammary glands as a negative control to test the specificity of the staininIn all our immunohistochemical analyses of PRLR in mouse mammary tissue, we used PRLR - deficient mammary glands as a negative control to test the specificity of the staininin mouse mammary tissue, we used PRLR - deficient mammary glands as a negative control to test the specificity of the staining.

Make sure to always perform the negative, eccentric part of the lift in a very slow and controlled manner, and allow for an equally slow and smooth transition to the positive, or concentric portion, but then lift the weight up explosively to recruit as many muscle fibers as possible.

Use full range of motion and perform both the positive and negative portion of the movement in a slow, controlled manner, and don't forget to squeeze the calves as hard as possible.

We know, for example, that factors such as negative mood, fatigue and alcohol play a large part in self - control failure.

However, additional, longer term (≥ 1 y), randomized controlled trials are needed in this area, including those that investigate the optimal dose of pulses for weight control balanced with other factors worth considering, such as any potential negative effects (e.g. gastrointestinal tolerance, phytate effects of mineral absorption).

The main benefits of Talk a Lot are: • Students have to think in English during lessons in a controlled and focused way • Students learn how to memorise correct English structures naturally, without abstract and unrelated grammar lessons • Students learn how to construct eight different common verb forms, using positive, negative and question forms, as well as embedded grammar appropriate to their level.

In contrast to the more negative connotation of the term dominance as forceful control or command over others, they define dominance as the teacher's ability to provide clear purpose and strong guidance regarding both academics and student behavior.

As an MRO, you are prohibited from doing the following as part of the verification process: (e) You must not verify a test negative based on information that a physician recommended that the employee use a drug listed in Schedule I of the Controlled Substances AcAs an MRO, you are prohibited from doing the following as part of the verification process: (e) You must not verify a test negative based on information that a physician recommended that the employee use a drug listed in Schedule I of the Controlled Substances Acas part of the verification process: (e) You must not verify a test negative based on information that a physician recommended that the employee use a drug listed in Schedule I of the Controlled Substances Act.

The negatives: cruise control is not standard (I added it), long trips are not as comfortable as most cars, the cupholders are positioned in front of the vents, blocking air flow from the dash and the steering wheel has an odd texture that is not very comfortable.

As she explained to Flood for the piece, which has been written for inclusion in The Guardian's ongoing series on self - publishing, the negative aspects such as loss of control over her covers and the publisher's insistence on squashing her work into pre-existing molds like chick lit or «misery lit» (whatever that may be), made her question the entire procesAs she explained to Flood for the piece, which has been written for inclusion in The Guardian's ongoing series on self - publishing, the negative aspects such as loss of control over her covers and the publisher's insistence on squashing her work into pre-existing molds like chick lit or «misery lit» (whatever that may be), made her question the entire procesas loss of control over her covers and the publisher's insistence on squashing her work into pre-existing molds like chick lit or «misery lit» (whatever that may be), made her question the entire process.

Practices may vary according to the type of financial information sought; the exclusion of items outside applicant's control, such as medical expenses; the time period preceding the employment application for that information; when in the selection process the information is used; and consideration of the applicant's explanation for negative information.

However, partaking in a debt consolidation program can also be seen as negative for the fact that your credit card debt is out of control.

In those circumstances, the dogs are already well trained, the owner is in agreement that we go this route and they have been instructed by me each step of the way, we are typically working on more distance control (such as when the dog is 100 yards or more away from the handler), the dog has been tested and isn't showing any negative effects, we are putting the final polish on solidifying each of the trained behaviors, and we have determined that this is the best course for the welfare of the doIn those circumstances, the dogs are already well trained, the owner is in agreement that we go this route and they have been instructed by me each step of the way, we are typically working on more distance control (such as when the dog is 100 yards or more away from the handler), the dog has been tested and isn't showing any negative effects, we are putting the final polish on solidifying each of the trained behaviors, and we have determined that this is the best course for the welfare of the doin agreement that we go this route and they have been instructed by me each step of the way, we are typically working on more distance control (such as when the dog is 100 yards or more away from the handler), the dog has been tested and isn't showing any negative effects, we are putting the final polish on solidifying each of the trained behaviors, and we have determined that this is the best course for the welfare of the dog.

My study of dog bite - related fatalities occurring over the past five decades has identified the poor ownership / management practices involved in the overwhelming majority of these incidents: owners obtaining dogs, and maintaining them as resident dogs outside of regular, positive human interaction, often for negative functions (i.e. guarding / protection, fighting, intimidation / status); owners failing to humanely contain, control and maintain their dogs (chained dogs, loose roaming dogs, cases of abuse / neglect); owners failing to knowledgably supervise interaction between children and dogs; and owners failing to spay or neuter dogs not used for competition, show, or in a responsible breeding program.

In opposition are animal advocates including the British singer Morrissey who are appalled at the rhetoric of a war on cats and promote nonlethal methods of controlling the negative effects of cats as being more effective and humane.

Given that a number of other animal control programs and regulatory changes were implemented during the study period, separating out the effects of a single program may be impossible... Data were not collected on how many adoptable versus nonadoptable cats were impounded, so it was not possible specifically to assess changes in outcomes for feral cats, only changes in overall cat outcomes... it can be stated that the implementation of sterilization of feral cats as part of a TNR program did not result in negative changes.

The game was criticised for some negative issues, such as lapses in the control system.

Despite some of the negatives with the controls, there are plenty of positives as Golden Abyss takes advantage of the slick handheld machine in so many ways.