Sentences with phrase «as a positive control»

Gladys Kaba from the University of Health and Allied Sciences in Ghana will be receiving the third Addgene / Seeding Labs plasmid grant and plans to use the grant to order plasmids she can use as positive controls in PCR - based cervical cancer screens.

Whole genome expression data of livers of wildtype and PPARα − / − mice fasted for 24 h served as positive control for PPARα - dependent gene regulation.

In contrast to repression by HP1, which served as a positive control for repression, the effect of ACF1 strongly depends on the chromatin context and is particularly robust in lowly expressed genes in overall inactive chromatin domains.

Second, they must have conducted the experiments adequately as demonstrated by pre-agreed quality checks, such as positive controls and manipulation checks.

«Histamines are used in skin and respiratory allergy tests as a positive control — they cause a bump in skin tests and restrict breathing in respiratory tests,» DeVries said.

The tests were done in three ways — with the retinal prosthesis turned on in a standard mode, in a scrambled mode as a positive control, and turned off as a negative control.

1H7 was raised from recombinant full - length AS, as was 9E4, a mAb previously used successfully to target AS in PDGF - AS mice [20] and was therefore included in the new study as a positive control for the 3 novel mAbs.

There was no significant increase in progranulin protein levels in the frontal cortex (Fig. 4B h), thalamus (Fig. 4C i), or hippocampus (Fig. 4D j), despite observing the expected increase in doublecortin - positive neurons in the dentate gyrus (Fig. 4E t), indicating increased neurogenesis as a positive control.

Using serial dilutions of a cloned fragment of XMRV gag as a positive control, the nested PCR assay could reliably detect at least 3 copies of DNA per reaction, even when spiked into genomic DNA prepared either from 293FT cells or donor PBMCs previously validated to be negative for XMRV.

We recommend using CoCl2 or DFO treated cells as a positive control.

The β - catenin / TCF binding site for c - MYC was amplified as a positive control, and the genomic sequence for β - actin was amplified as a negative control.

in vitro culture of human nerve cells (yellow; nuclei: purple) exposed to various concentrations of different flame retardants: PBDE 99 is already in use and suspected to be harmful to health; the substance was thus used as a positive control.

One reaction serves as a positive control to amplify a portion of the tubulin gene found in all plants, and the other assays for genetic modification.

Adenovirus PCR was performed on a TMAdV - positive clinical sample, a TMAdV culture, and a human adenovirus B culture (as a positive control) using an additional 5 pairs of primers, according to previously published protocols [26], [27], [28] Three of the 5 primer pairs, designed to detect human respiratory adenoviruses B, C, and E, failed to amplify TMAdV [27].

11 and our observations), stimulated with 10 ng / ml TNF - α, served as positive controls.

GAPDH was used as a positive control.

Expression of GAPDH was used as a positive control.

Antibodies with an uncertain routine WB have been revalidated using an over-expression lysate (VERIFY Tagged Antigen (TM), OriGene Technologies, Rockville, MD) as a positive control.

Inhibition of miR - 181a resulted in induction of Smad signalling and led to the promotion of neurite growth in these cells, in a similar fashion to that induced by the physiological ligand, GDF5, which was used as a positive control.

As positive controls, we measured hippocampal BDNF and neurogenesis.

Given that BMPR2 and ACVR2A were predicted and validated targets of miR - 181a (Table 2), the cells were treated with GDF5 (also known as BMP14) as a positive control, because GDF5 is known to bind BMPR2 or ACVR2A and activate Smad signaling [28].

The benzo [a] pyrene (BaP)- induced p53 expression was used as a positive control, and the blue fluorescence dye, Hoechst 33342, revealed the location of the nuclei.

Genomic DNA from HB1.F3.H2IgG cells was used as a positive control.

Aurora kinase A (AURKA), a known transcriptional target of FoxM1 (Lefebvre et al., 2010), was used as a positive control.

Primers for the promoter region of CDC25B were used as a positive control.