Sentences with phrase «as cell sorting»

By combining advanced approaches such as cell sorting and single cell analysis, researchers will gain a better understanding of the complex genetic landscape of disease.

Not exact matches

We anticipate some sort of growth toward increased complexity: increasingly larger organic macromolecules, then the convergence of many macromolecules to constitute a simple living system, either as a cell with its protective wall and vital nucleus or as some functional analogue, then the convergence of many cells to form larger organisms.

As a physics teacher, I teach the therapeutic effects of gamma radiation in radiotherapy, along with the associated dangers (radiation can cause cells to become cancerous as well as kill cells that are already cancerous), but a common misconception among students is that cancer cells are rather like viruses or bacteria, a sort of alien cell that has entered the body, growing out of control with little relation to the surrounding cellAs a physics teacher, I teach the therapeutic effects of gamma radiation in radiotherapy, along with the associated dangers (radiation can cause cells to become cancerous as well as kill cells that are already cancerous), but a common misconception among students is that cancer cells are rather like viruses or bacteria, a sort of alien cell that has entered the body, growing out of control with little relation to the surrounding cellas well as kill cells that are already cancerous), but a common misconception among students is that cancer cells are rather like viruses or bacteria, a sort of alien cell that has entered the body, growing out of control with little relation to the surrounding cellas kill cells that are already cancerous), but a common misconception among students is that cancer cells are rather like viruses or bacteria, a sort of alien cell that has entered the body, growing out of control with little relation to the surrounding cells.

Called SIF - seq, for site - specific integration fluorescence - activated cell sorting followed by sequencing, this new technique complements existing genomic tools, such as ChIP - seq (chromatin immunoprecipitation followed by sequencing), and offers some additional benefits.

Biofilms are made up of the bacterial cells as well as all sorts of other material — carbohydrates, proteins, and so on — that the bacteria build to protect themselves.

The exceptional cancer cells that escape the astrocytes do so by producing a protein called Serpin, which acts as a sort of antidote to the death signals fired at them by nearby astrocytes.

When dendritic cells not only signal myelin as a «target substance,» but also secrete the messenger substance IL - 6, a sort of molecular switch is flipped in the T cells.

Using a powerful microscope to observe mouse oocytes as they split, Ellenberg's group found that the spindles assembled into two coherent structures, one for the future egg and one for the future polar body.At first, spindles appeared throughout the cell in a sort of mesh.

Those qualities make graphene a tantalizing alternative for use as a transparent conductor, the sort now found in everything from computer displays and flat panel TVs to ATM touch screens and solar cells.

CTL1 is also found in animal cells, Chao noted, and thus the study concluded that «characterizing CTL1 as a new regulator of protein sorting may enable researchers to understand not only ion homeostasis in plants but vesicle trafficking in general.»

Depending on which device they buy, researchers can photograph cells at anywhere from 20x to 60x magnification, and use the included software to sort them by purely visual characteristics such as shape and size, fluorescent markers, or both.

The specialized immune - system function of dendritic cells is to sample proteins and serve as a sort of security guard, sorting out alien proteins from the home team.

So if you think about the egg as sort of a uniform ball, you put a messenger RNA on one end of the egg, and when you cut up the ball, then only the cells at that end get that information.

In the last few years, researchers have learned how to turn embryonic stem cells into all sorts of different cell types, such as skin cells, heart

Such methods can be designed to sort cells by size or to identify cells that display a targeted feature, such as a fluorescent dye that has been added.

Using a high - speed actuator as the driving source of pump, they succeeded in producing a flow with 16 microseconds for cell sorting.

The researchers also found that an enzyme called DGAT1 (diacylglycerol acyltransferase) is crucial to the re-esterification process, acting as a sort of cell police officer to ensure that toxic fatty acids stay away from the ER.

The bone matrix being produced by cells is stained red in samples of cells that are sorted for not expressing ALPL (left), sorted for expressing ALPL (right), and left as an unsorted control (center).

Still others gain entry by disguising themselves as the sort of free - floating molecules that our cells routinely gobble up.

An antibody that works well for Western blots on denaturing gels, for example, should not be presumed to work equally well in nondenaturing assays such as fluorescence - activated cell sorting.

Two years ago, Janelia researchers reported that the flies appear to have a group of about 50 cells connected in a sort of ring in the center of their brains that serve as an internal compass.

The device also prevents the type of damage to cells that can occur with traditional sorting methods, such as those using high - force centrifuges.

These DNA strands act both as a sort of molecular Velcro and as a bar code that specifies where each cell belongs within the organoid.

«It's the same process as for single cell genomics, but for aggregates of symbiotic bacteria and archaea,» said DOE JGI Microscale Applications Group head Rex Malmstrom of the technique called BONCAT - FACS (BONCAT — Fluorescence - Activated Cell Sorticell genomics, but for aggregates of symbiotic bacteria and archaea,» said DOE JGI Microscale Applications Group head Rex Malmstrom of the technique called BONCAT - FACS (BONCAT — Fluorescence - Activated Cell SortiCell Sorting).

In addition, they serve as a sort of internal repair system in many tissues, dividing essentially without limit to replenish other cells.

The mitochondrion is an organelle that produces the energy needed for the cell survival, operating as a sort of power station.

In a healthy person bone marrow acts as a sort of factory of stem cells, the vital cells that mature and divide to form other essential blood cells throughout the body.

New research at Rockefeller University, published March 18 in Nature, reveals how cells sort out the loops meant to encode small RNAs, known as microRNAs, by tagging them with a chemical group.

«Our hypothesis is that maybe this sort of intrinsic mechanical property — the friction these proteins experience — helps them to get where they need to be, as well as maintain the spindle's organization and mechanical integrity during cell division,» Forth says.

Lysosomes act as a sort of recycling and disposal center for cells.

They feed on the bottom of the food chain — on single - celled plankton, which larger fish can not eat — and then they become prey for all sorts of upper - level predators like tuna, sea bass and halibut as well as seabirds and marine mammals.

«A microfluidic device that sorts cells based on their mechanical properties could offer cost and labor advantages over current methods and may provide sufficient enrichment to serve as an alternative or additional approach to antibody - based techniques,» Sulchek says.

Because the treatment uses the patient's immune cells as a sort of T - cell training force, it is an immunotherapy.

Many things are being secreted, which the brain sort of modulates, like the defensins from Paneth cells, there are many endocrine cells, serotonin cells in the gut, enterochromaffin cells, which are under autonomic control, so that we now know that serotonin, as well as norepinephrine, can, under stress for example, increase in the lumen of the gut.

Current efforts, mostly conducted in animal models, involve attempting to derive and aggregate embryonic stem cells, exposing them to such factors as acetic acid, allowing them to differentiate, or specialize, and then sorting through these cells to extract the cell types of interest.

BTHP - 1 parental cells were transduced with the MLV vector MX - DC - SIGN and FACS sorted as a population for high levels of DC - SIGN expression.

We delve into very fundamental problems such as «how does a malaria red blood cell attach itself to a blood vessel» or «how does binding of a ligand to a cell surface receptor or contact of a crystalline surface with the plasma membrane drive lipid sorting and how will this lead to signalling» but then immediately apply it to a practical problem such as «how does contact of uric acid crystals with dendritic cells cause gout in affected joints and how can we prevent this occurrence?»

We were then able to sort through both sets of sequences and detect mutant cells — even when they made up as little one in every thousand cells.

Human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), are known to be vulnerable to apoptosis upon various technical manipulation, such as single cell dissociation, freezing and thawing, etc., which hinder their use for clonal isolation in gene transfer, differentiation and FACS cell sorting.

Format: Website Years Covered: currently active UK trials as of June 2017 Search Strategy: Not disclosed Project Focus: advanced cell therapy, active that year, in the UK only Special Notes: on - line sort by 4 parameters, archives for previous years back to 2012.

Since very few studies have demonstrated the formation of macro-metastasis from low numbers of sorted CSCs and currently proposed CSC markers might not be sufficient to identify all stem cell populations [13], we believe that our study presents a promising approach to isolate stem - like and metastatic breast CSCs as opposed to the cell - sorting strategy based on putative stem cell surface markers.

However, as demonstrated by a recent report from Stuelten and colleagues [13], the complexity of CSC markers continues to pose challenges for identifying and isolating the putative tumor stem cell populations by the cell - sorting approach.

(C) Sorted Lin - CD127 + cells from Rag1 - / - splenocytes were pretreated with DMSO or indicated concentrations of PD98059 or SB203580 as in A and analyzed for IL - 22 secretion by ELISA.

At various points in this process, cells use RNA as a sort of scaffold to help replicate DNA.

As these are very rare cells, we first expanded primary sorted ILCs in vitro to obtain larger numbers of ILCs (S4 Fig).

In a study using human muscle tissue, scientists in Children's Stem Cell Research Center - led by Johnny Huard, PhD, and Bruno Péault, PhD - isolated and characterized stem cells taken from blood vessels (known as myoendothelial cells) that are easily isolated using cell - sorting techniques, proliferate rapidly and can be differentiated in the laboratory into muscle, bone and cartilage ceCell Research Center - led by Johnny Huard, PhD, and Bruno Péault, PhD - isolated and characterized stem cells taken from blood vessels (known as myoendothelial cells) that are easily isolated using cell - sorting techniques, proliferate rapidly and can be differentiated in the laboratory into muscle, bone and cartilage cecell - sorting techniques, proliferate rapidly and can be differentiated in the laboratory into muscle, bone and cartilage cells.

The core facility offers assisted cell sorting services for characterizing, separating and purifying populations of particles as diverse as beads, micro-particles and cells.

For mouse ILC3s, splenocytes from Rag1 - / - mice were sorted (Lin -(CD3, CD45R, CD11c, GR - 1, NK1.1) c - kit + Thy1.2 +) and cultured as described for human cells using recombinant mouse cytokines.

Indeed this sort of model has been used to explain the existence of a stable attractor and associated lineage primed states in EML cells, a haematopoietic progenitor cell line [54], and as the basis for heterogeneity in Nanog expression in ES cells [11].

While no one can say with certainty what sort of life might be turned up by these experiments, the usual assumption is that it will be microbial, as single - celled life is adaptable to a wide range of environments and requires less energy.