Sentences with phrase «as confocal»

The team has systematically validated the fidelity, accuracy, reliability, reproducibility and versatility (for many cell types) of this method, using various other techniques such as confocal microscopy, flow cytometry, Western blotting and Raman spectroscopy.

But subtler changes require higher - resolution approaches, such as confocal or electron microscopy.

The chip is compatible with optical imaging technologies such as confocal microscopy, allowing the researchers to obtain high - resolution images and movies of the fertilization process, as it occurs.

The research team is now exploring additional biological applications for the new system and is working to extend the method to other microscope modalities, such as confocal microscopy.

Companies making high - content cell imaging equipment such as confocal microscopes and plate readers are now adding software tools to process images of 3D cell cultures.

Not exact matches

«Moving to non-invasive and repeatable methods of nerve fiber measurements such as in vivo corneal confocal microscopy would enhance study of peripheral neuropathy by enabling early detection of damage, progression of nerve fiber deterioration, and enable assessment of therapeutic strategies in the SIV / macaque model,» explains Dr. Mankowski.

Together, these findings demonstrate that emerging noninvasive techniques to measure corneal nerve fiber alterations such as in vivo corneal confocal microscopy may be useful clinical tools to screen for and monitor progression of peripheral neuropathy in HIV - infected patients.

As someone who has trouble working a washing machine, I consider it a personal achievement that I am able to use a confocal microscope, run a Western blot, and carry out a DNA replication assay!

Using chemicals like these in combination with new imaging tools, such as the multi-photon confocal microscope, has enabled researchers to explore the minuscule world of the neuron and observe brain cells in action with far more precision.

GET YOUR GLOW ON The reef coral Montipora capitata naturally fluoresces in red and cyan, as seen in this confocal microscopy image.

Acquiring images using modern techniques such as light sheet fluorescence, confocal, or electron microscopy creates a significant data stream.

In addition to fruit flies, they successfully used the program to analyze images of zebrafish and mice, as well as data collected from a commercial light sheet microscope and a commercial confocal microscope.

Another popular technique for 3D cell imaging called spinning disc confocal microscopy can't image samples nearly as long as Betzig's technique, but it is still better for imaging thick cells and tissue.

So the Bessel beam can scan a cell with the same total flux as the light spots used in confocal microscopy, but that flux is distributed among the seven beams and the cell isn't flooded with excess light; thus, the cell isn't damaged nearly as quickly.

Although traditional in vivo imaging tools, such as widefield and confocal microscopy, and newer ones, such as light - sheet microscopy, can image in three dimensions, they sacrifice substantial spatiotemporal resolution to do so and, even then, can often be used for only very limited durations before altering the physiological state of the specimen.

«Nonlinear stage - scanning confocal microscopy is critical because it allows us to rapidly measure the nonlinear emission from thousands of different nanostructures while minimizing the potential systematic errors, such as intensity or beam pointing variations, often associated with tuning the wavelength of an ultrafast laser,» O'Brien says.

In the first type, which was conducted at Rice, the team used confocal microscopy to film «giant unilamellar vesicles» (GUVs), synthetic membrane - enclosed structures that are about the same size as a living cell.

Druckenmiller houses a specialized laser scanning confocal microscope (the only one of its kind in the state of Maine), as well as a scanning electron microscope and a number of conventional fluorescent microscopes, all of research quality.

The IHC was done following the same protocol as for the confocal experiments, except that the McSA1 and pab27576 were used at a concentration of 1:1000.

In this webinar, the principles of state - of - the - art confocal Raman imaging as a tool for the analysis of molecular characteristics of a sample will be presented, then the manner in which this information can be linked to structural information acquired with scanning electron microscopy will be demonstrated.

Vector is also designed for high - speed scanning imaging techniques such as multiphoton imaging or laser scanning confocal microscopy.

The researchers used the techniques, such as fluorescent correlation spectroscopy and confocal microscopy, for measurements of protein - mineral binding.

Experience in Confocal and Super-resolution microscopy as well as image analysis is highly desireable.

We work across disciplines and use a variety of techniques including microfluidics, standard microscopies (electron, optical, fluorescence, confocal), spectroscopies (fluorescence, UV, CD), scattering techniques (X-ray, light), protein expression and characterization and cell - free gene expression to investigate the utility of coacervate microdroplets as robust reaction compartments and cellular mimics.

In this course, trainees will perform experiments involving both classical physiological models, as well as modern reductionist approaches and confocal microscopy to follow trafficking of transporter proteins in cultured cells.

The lab regularly uses techniques such as dye backfills, immunohistochemistry, in situ hybridization, PCR, and confocal microscopy.

Among vertebrates, the fish model displays easy high density breeding (short generation time), favourable biological peculiarities (accessibility and transparency of the eggs, genome sequenced for both species used on the platform), original technological approaches (confocal and two - photon microscopy), as well as a wide accessibility to fully transparent larvae usable up to 5 days after fertilization.

Overcoming the limitations of confocal fluorescence microscopy, we invented and developed light sheet - based fluorescence microscopy (LSFM) establishing it as a major tool in the modern life sciences.

There, she managed a large number of confocal, advanced light, transmission, and scanning electron microscopes, as well as image processing and bioinformatics.

Caroline joined the Biophotonics group as a post doc in 2013 to implement confocal light - sheet microscopy for structural whole mouse brain imaging and then later functional calcium imaging in Zebrafish.

Traditionally, this type of sample is imaged with a parallelized confocal technique such as spinning disk confocal.

As an appropriate ECM substrate for cancer cell migration, we use nonpepsinized rat tail collagen I polymerized at room - temperature and fluorescently labeled to facilitate visualization using standard confocal microscopes.

Fluorescence confocal microendoscopy has the capability to image the epithelial and subepithelial layers of the proximal bronchial tree, as well as the more distal parts of the lungs, from the terminal bronchioles down to the alveolar ducts and sacs.

Functional imaging using in vivo confocal microscopy which allows the analysis of vaso - activity phenomena during hypoxia, of ischaemia reperfusion events, or of homing cells in pathologic processes, such as tumours or inflammatory diseases.

Using a laser scanning confocal microscope, we have examined positions of the genomic loci in the 3D nuclear compartment at as short as 1.5 - second intervals.

In the paper, the authors write: ′ As the present study shows, the combination of light microscopy, scanning and transmission electron microscopy and three - dimensional confocal laser microscopy, successfully contributed novel information on the structure and morphology of ceratomyxid parasite stages in the bile, and provided unique insights into parasite composition, cell motility and cytokinesis in myxozoans, which had not previously been studied.

′ The researchers point out that although confocal CLSM may be a poorly used tool, it is extremely useful for investigating the three - dimensional morphology of the parasites as well as for determining the presence and location of certain cellular components.