Sentences with phrase «as donor dna»

Led by Dr. Peter Glazer, chair of therapeutic radiology, Mark Saltzman, chair of biomedical engineering, and Dr. Marie Egan, professor of pediatrics and of cellular and molecular physiology, the collaborative team used synthetic molecules similar to DNA — called peptide nucleic acids, or PNAs — as well as donor DNA, to edit the genetic defect.

If you are looking to insert small DNA sequences (< 200bp), using a DNA oligonucleotide as donor DNA should do the trick.

Led by Dr. Peter Glazer, chair of therapeutic radiology, Dr. Mark Saltzman, chair of biomedical engineering, and Dr. Marie Egan, professor of pediatrics and of cellular and molecular physiology, the collaborative team used synthetic molecules similar to DNA — called peptide nucleic acids, or PNAs — as well as donor DNA, to edit the genetic defect.

Not exact matches

Now cells from two one - year - old babies born as a result of this treatment have indeed turned out to have a little extra DNA from a donor mother, as well as that from their own parents.

For editing the genome, this system makes use of 3 components, a guide RNA (gRNA) of about 125 nt that specifies the target, the Cas9 endonuclease that creates the DNA double - strand break (DSB) at the target site, and a donor oligonucleotide or plasmid as the repair material if needed (for knock in models).

The idea here is that the donor DNA acts as a DNA repair template that will be inserted at the DSB during homologous recombination (HR).

Three human cornea pairs (donor ages 30 - 37 years), stored in Optisol, were labeled by chemifluorescence to show incorporation of BrdU into new DNA using a commercial procedure (Hoffman - La Roche, Nutley NJ) as based on the work of Elwart and Dormer [21].

Total RNA from Cellartis enhanced hiPS - HEP cells from C12, C18, and C22 (n = 2 batches per cell line) was extracted on Day 13 post-thawing as well as from hphep cells (n = 3 donors) Day 1 post-thawing using the GenElute RNA / DNA / Protein Plus Purification Kit (Sigma Aldrich).

The technical evaluation of projects may require the provision of additional data such as information on the genetic modification of your mutant mouse line if applicable (e.g. affected gene, MGI ID of the gene, type of mutation, ES - cell line used, genetic background (e.g. number of backcross generations), safety level, description of DNA modification, vector, remaining non-recipient DNA, donor organism), mutant phenotype (s), special housing or care requirements, current sanitary status, and intellectual property rights (who generated the mouse line, owner of the mouse line)

Using serial dilutions of a cloned fragment of XMRV gag as a positive control, the nested PCR assay could reliably detect at least 3 copies of DNA per reaction, even when spiked into genomic DNA prepared either from 293FT cells or donor PBMCs previously validated to be negative for XMRV.

B. Semi-quantitative β - actin PCR results for PBMC DNA specimens above in lanes 1 - 14; lane 15, water control; lanes 16 - 19, 10-fold dilutions of blood donor PBMC DNA starting at 0.1 ug as a positive assay control.

Our cumulative epidemiological and molecular data suggests a recent switch of the O - AGC between isolates with O156: H8 strains having served as DNA donors.

Testing on DNA of sperm found on the child's underwear worn during the rape excluded James Bain as the donor of the sperm, confirming that someone other than Bain raped the victim.