hESC lines I6 and H9 were maintained on Matrigel (BD Biosciences, Bedford, MA; http://www.bdbiosciences.com) coated dishes in medium (comprised of Dulbecco's Modified Eagle's Medium / Ham's F12 supplemented with 20 % knockout serum replacement (KSR), 2 mM non-essential amino acids, 4 mM L - glutamine, 0.1 mM β - mercaptoethanol, 50 µg / ml Penn - Strep, and 4 ng / ml of basic fibroblast
growth factor) conditioned with mouse
embryonic fibroblasts for 24 hours
as previously described [9], [10].
* The role of the US in global efforts to address pollutants that are broadly dispersed across national borders, such
as greenhouse gasses, persistent organic pollutants, ozone, etc...; * How they view a president's ability to influence national science policy in a way that will persist beyond their term (s),
as would be necessary for example to address global climate change or enhancement of science education nationwide; * Their perspective on the relative roles that scientific knowledge, ethics, economics, and faith should play in resolving debates over
embryonic stem cell research, evolution education, human population
growth, etc... * What specific steps they would take to prevent the introduction of political or economic bias in the dissemination and use of scientific knowledge; * (and many more...)