Sentences with phrase «as isoforms»

These varying combinations lead to the generation of slightly different proteins known as isoforms, which can end up functioning differently from each other.

We are one of the most referenced companies in the industry and continue to collaborate to develop products such as isoform - specific AKT antibodies, green fluorescent protein (GFP) and red fluorescent protein (RFP) antibodies, and fluorochrome - conjugated secondary antibodies.

Not exact matches

Oocytes contain a high concentration of an oocyte - specific isoform of p63 which plays a key role as a quality control factor in causing infertility.

In those cells the Esrp1 and Esrp2 proteins enforce epithelial - specific splicing isoforms for hundreds of different genes, as Carstens and his team have shown in studies since 2009.

These changes in mitochondria - and calcium - related markers occurred without any changes in the myosin heavy chain isoform composition of these muscles, as assessed by quantitative PCR (qPCR)(data not shown).

Adding to the documented complexity of IL - 15 — related activity, multiple isoforms and splice variants of IL - 15Rα exist as both membrane - bound and soluble forms that can either inhibit or potentiate IL - 15 biological activity (29 — 33).

Although the current study demonstrates that loss of IL - 15Rα promotes this phenotype, future studies are needed to determine the roles of specific IL - 15Rα isoforms in muscle, as well as the associations of additional IL15RA polymorphisms in muscle phenotypes in humans.

According to the C. elegans nomenclature, we suggest to rename W01A8.1 as Cel - plin - 1 (isoform a, b, and c) and proteins Cel - PLIN - 1 (isoform a, b, and c).

The GFP - tagged isoform a (plasmid pCK28 -LCB- PW01A8.1:: W01A8.1 (a) synth:: gfp:: unc - 54 3 ′ UTR -RCB--RRB- was constructed by synthesizing the W01A8.1 a sequence with modified codons to allow protection from CRISPR / Cas9 targeted sgRNA and prepared as a GeneArt ® Strings ™ DNA Fragment from Invitrogen (Invitrogen, Carlsbad, California, USA) and cloned using GeneArt ® Seamless Cloning System (Invitrogen) into pPD95.75 (NeoR).

(D) Diagrammatic image of four transcript isoforms that were detected as alternative splice variants resulting from a SNP in the 2nd intron of bsl1 - 2.

This figure depicts how the expression of the longer transcript in this gene becomes less dominant as PMI increases, and, as a consequence, the abundance of the different isoforms tends to converge

As a proxy for splicing alteration, we computed the Shannon's entropy on the relative abundance of a gene's alternative splicing isoforms (Methods)-- higher values corresponding to more stochastic production of alternative isoforms.

The usage of a major isoform across tissues and biological conditions has been reported as a general characteristic of genes38, 39, and has lead to extensive debate on the physiological relevance of regulated alternative splicing39.

Calculate isoform ratios for each gene: For a gene G, with k isoforms I, the splicing ratio is defined as:

Because the polyT repeat is reported as the key variant to define TOMM40 clades A and B, 12 we used this marker and APOE isoform information to perform analyses based on phylogenetic groups as described by Roses et al. 12 Haplotype phase was estimated using PHASE software.22 The phylogeny, which represents the evolutionary relatedness of the haploytpes, was estimated using neighbor - joining with 10 000 bootstrapping replicates in the CLC DNA workbench (CLC bio, Aarhus, Denmark)(Supplemental Figure 3; http://neuroscienceresearch.wustl.edu/Pages/cruchaga2011.aspx).

The ΔN isoforms of p63 may also serve as stem cell markers in a variety of epithelial cell types, including endometrium [30], cervix [31], breast [32], prostate [33], and in stratified squamous epithelium [21,34].

This could be interpreted as UNC - 7L and UNC - 9 forming heteromeric channels, with ion selectivity properties that are influenced by both isoforms (as this dictates the rectification of Gj0), but Vj gating properties dominated by UNC - 7L (as this dictates the shape of the Gj ∞ plot).

In contrast, the ΔNp73 isoforms act as dominant negative inhibitors and can even promote cell immortalization [44].

It also suggests that in unc - 9 mutants, the native UNC - 7 isoforms expressed in motor neurons (as detected by previous analysis of total UNC - 7 expression) are insufficient to properly localize AVB - expressed UNC - 7S:: GFP (that is, UNC - 7 in motor neurons does not substitute for UNC - 9 in localizing AVB - expressed UNC - 7S).

Polycistronic cassettes that contained the KLF4L isoform had more robust initiation of reprogramming (evaluated by an alkaline phosphatase - positive assay) as well as stabilization (Nanog - GFP reporter activation and silencing of factor - linked mCherry).

Cortisone is produced by the action of 11β - hydroxysteroid dehydrogenase (11β - HSD), an enzyme with two isoforms, the first of which, 11β - hydroxysteroid dehydrogenase I (11β - HSD I) catalyzes cortisone into cortisol, enabling rapid supply of the active hormone as needed.

Rimando A. Pterostilbene as a new natural product agonist for the peroxisome proliferators - activated receptor alpha isoform.