Sentences with phrase «as microinjection»
«Conventional techniques of producing transgenic animals, such as microinjection of genes into eggs and the retroviral transduction of genes into embryos, often produce many animals that are mosaic, which means they do not contain the foreign gene in all their cells.
https://www.genome.gov/ Not exact matches
Microinjection of wild - type human ACVR1 RNA into alk8 — / — zebrafish embryos rescued approximately 80 % of the injected embryos completely or partially (Figure 3, C and F), showing that human ACVR1 can function as a BMP type I receptor in this zebrafish model and substitute for Alk8.
Transgenic lines were prepared using microinjections into gonads of young adult N2 hermaphrodites as described (Tabara et al., 1999; Timmons, Court & Fire, 2001; Vohanka et al., 2010).
The level of fluorescence you saw in the validation assay should give an accurate indication of how many pups will contain a mutation and many of the mice with the mutation will be homozygous for it in every cell; however, if the microinjection was done as the pronuclei were fusing, you may have several mosaic mice.
When overexpressed together in developing Xenopus embryos by RNA microinjection, these genes can induce eye - like structures as defined by the expression of markers for some retinal cell classes [7].