Sentences with phrase «as single sequences»

Small moments in the other districts work to create tension and suggest a build up to a big battle, but that's because the military strikes and executions are done as single sequences, rather than intertwined with the rest of the story.

Viewed as a single sequence, therefore, unrest at Campi Flegrei can be explained by the evolving deformation of an elastic - brittle shallow crust.

Not exact matches

The chase sequence involving an elaborate single take stands out as one of Spielberg's greatest action set pieces.

The goal here is to use «single - cell sequencing to understand how many different cell types there are in the human body, where they reside, and what they do,» as Nature reports.

But I do see the divine priority, God's prevenient guidance, in the event as a whole — the history of Old Testament Israel, the birth, ministry, death and resurrection of Jesus, and the coming into being of his church — and in its effect, which we variously describe as the supreme revelation of God's love, the redemption of the world, the coming into being of the church.15 Indeed it is precisely God's prevenient guidance that makes of this entire historical sequence, including its climax in Jesus, one single event, producing one single effect.

They are not in great form out on the road at the moment having lost their last six away games as well without scoring a single goal in that sequence of matches.

Using a combination of old - fashioned clinical observation and modern biochemical analysis, he has shown that a person's appetite and their eating behavior can be linked to specific genes — and that even a tiny defect such as the absence of a single nucleic acid in a sequence of DNA can lead to runaway weight gain.

Using a technique known as single - cell RNA sequencing, the team explored more than 65,000 individual cells that exist under normal or inflammatory conditions, looking for genes that were more active in one state or subpopulation versus another.

No one is arguing that Lamarck got everything right, but over the past decade it has become increasingly clear that environmental factors, such as diet or stress, can have biological consequences that are transmitted to offspring without a single change to gene sequences taking place.

«Whole - genome, single - cell [sequencing] costs about the same as bulk - cell sequencing,» says BGI's Peng.

Fully sequenced genomes remain rare, so the bulk of the analysis was done by looking at genetic markers known as single nucleotide polymorphisms or SNPs.

As costs come down and methods become more standardized, experts in the field expect single - cell sequencing to become the new model for both transcriptomic and genomic studies.

Some also offer the technique as a service, taking customers» vials of cells and returning complete single - cell RNA sequence data.

«Whole - genome, single - cell [sequencing] costs about the same as bulk - cell sequencing.»

If the speed of advancement in this area continues apace, even some of the more formidable challenges, such as single strand sequencing and the $ 1,000 genome, might be overcome — not just in our lifetimes, but within the foreseeable future.

As equipment makers and service providers try to make single - cell sequencing accessible to more labs, scientists at the forefront of the field are pushing the technique into entirely new territory.

Complicating matters further is the fact that, as the field matures, researchers need to collect and analyze sequences from multiple specimens to prove that their results are not based on the idiosyncrasies of a single sample.

This RNA snippet, known as sgRNA (single guide RNA), contains a sequence of 20 RNA - letters complementary to the genomic target site that scientists have hitherto had to select laboriously by hand or with a variety of online tools.

Even if we assume that a trillion trillion trillion trillion unique sequences 180 nucleotides long could function as RNA replicases, the probability of hitting any one of them in a single shot is still only about 1 in 1060.

The sequenced genome of a single individual yields about half a terabyte of data of information — that's about as much information as you'll find on 106 DVDs.

Described in the January 7th issue of Neuron, the technique uses «deep,» highly sensitive whole - genome sequencing of single neurons and a new technology that identifies inserted bits of DNA caused by retrotransposons, one of several kinds of so - called somatic mutations that can arise as the brain develops.

In response, Page and colleagues devised what is known as SHIMS (single - haplotype iterative mapping and sequencing) to establish a definitive reference DNA sequence of the Y chromosome.

Navin's team developed Nuc - Sec as a single - cell genome sequencing method and applied it to study how cell mutations occur in both types of breast cancer.

«By sequencing all of the DNA that codes for mRNA and ultimately, proteins, Dr. Amin and colleagues found a single gene that may account for as much as 4 % of the heritable risk for depression,» said Doctor John Krystal, Editor of Biological Psychiatry.

With the completion of the first phase of the Human Genome Project in 2000, and the advent of sequencing technologies that can detect gene variations such as single nucleotide polymorphisms (SNPs), for the first time scientists have the tools in hand to find the key immune genes and genetic networks that play roles in vaccine response.

Existing technology, known as next - generation sequencing (NGS), measures genomes derived from millions of cells versus the newer method for single - cell sequencing, called Monovar.

The third is a method implemented by Reich for reading the genetic codes of 1.2 million carefully chosen variable parts of DNA (known as single nucleotide polymorphisms) rather than having to sequence entire genomes.

The study used whole - exome sequencing to characterize genetic alterations that occur at the single nucleotide level for all genes in 25 cell lines commonly used as models of bladder cancer.

The researchers compared the dogs» DNA, looking for sequences that differed by a single base, known as single - nucleotide polymorphisms.

«Sniffing out stem cell fates in the nose: Using single - cell RNA sequencing and clever statistical analysis to track stem cells as they mature.»

The DNA sequences of HIV particles in a single person can be as diverse as those of all the influenza viruses in the world.

Meyer's breakthrough came in developing a method to start the sequencing process with single strands of DNA instead of double strands, as is usually done.

The team also compared the 7442 genes found as single copies in both the cobra and the python with the same genes in all other land vertebrates sequenced so far.

Through a combination of single - molecule imaging and bulk biochemical experiments, the research team has shown that the genome - editing ability of Cas9 is made possible by the presence of short DNA sequences known as «PAM,» for protospacer adjacent motif.

«Once precisely assembled with a specific base sequence, these combined strands can be reproduced in a biotechnological process, as with single strands of bacteriophage DNA,» says Dietz, explaining the key feature of the process.

Using methods such as single cell sequencing, proteomics, and microscopy will allow exploration of this invisible and important world with great accuracy and depth.»

If you took high school biology in the 1990s, you probably learned about the molecular basis for human genetic disorders such as cystic fibrosis (1989), Huntingtons (1993), Duchenne and Becker muscular dystrophy (1987), and a rapidly growing list of single - gene disorders, and the correspondingly rapid growth in clinical diagnostic technology based on DNA sequence information, enabling certain diagnosis, sometimes before the advent of overt symptoms.

This includes the development of advanced laboratory tools such as CRISPR genome - editing and single - cell sequencing technologies.

Instead of using double - stranded DNA to make genetic libraries that can be fed into a next - gen sequencer, as was standard practice at the time, Meyer and his team first separated the double helix, then prepared the sequence library using each of the single strands, doubling the amount of fragments the group had to sequence.

Sequencing accelerates the analysis of cancer associated alterations in genome sequence such as insertions and deletions, CNVs, inversions, duplications, translocations and gene fusions, as well as single nucleotide variants.

• Mutation screening The unit can provide custom made assays for a wide range of known mutations associated with disease, such as single point mutations, insertions / deletions and sequence amplifications.

Using a combination of next - gen sequencing and a third - generation, single - molecule sequencer from Helicos Biosciences that can sequence DNA directly without the need for an amplification step, his group sequenced the 700,000 - year - old horse DNA that still holds the record as the oldest genome yet sequenced.4 «This type of technology will be the future,» he says.

Whole swathes of diagnostic tests may eventually be replaced with a single assay - sequencing - as we reach the «sequencing singularity».

As a response to the growing demand for single cell sequencing projects, in July 2016, the Single Cell Facility at the Hubrecht Institute was fosingle cell sequencing projects, in July 2016, the Single Cell Facility at the Hubrecht Institute was foSingle Cell Facility at the Hubrecht Institute was founded.

The researchers show that if the number of cells aggregated initially is similar to that of a mouse embryo, the cells generate a single axis and this serves as a template for a sequence of events that mimics those of the early embryo.

High - throughput single - cell RNA sequencing recently emerged as a powerful tool to profile complex and heterogeneous cell populations.

The goal of this project is to identify the specific nucleotide sequences and / or single nucleotide polymorphisms that are correlated with expression of virulence and disease via comparative genomic analysis between B. pseudomallei strains as well as between various bacteriophages harbored within B. pseudomallei.

To avoid these problems, the team used sequence data to create a list of single DNA letter changes, known as SNPs, which can be reliably identified in the gene - rich areas of the genome.

All variant loci were simulated with single - end reads using the genome sequence to build the reads, and for coding variants we simulated splice junctions and paired - end reads as well.

Stuart Lindsay, Ph.D., Arizona State University, Tempe, Ariz. $ 550,000 (3 years) «Molecular Reading Head for Single - Molecule DNA Sequencing» Building on the concept of threading DNA through a molecular pore, this group is developing a system in which a chemical ring, acting as a reading head, is used to measure differences in friction as DNA passes through the ring.