Ultimately, this week's discourse will lead to a consensus statement providing some guidance on how to approach using this and older gene editing technologies such
as zinc finger nucleases and enzymes called transcription activator - like effector nucleases, or TALENs.
Not exact matches
In recent years several techniques, such
as CRISPR / Cas9 or
zinc finger nucleases have been experimented to directly modify the DNA of plants and animals.
«Before CRISPR, there was TALENS [transcription activator - like effector
nucleases] and
zinc finger nucleases — older technologies that were not
as precise or reliable,» explains Sosa.
Although the study looked at CRISPR - Cas9 gene editing, the researchers believe their findings extend to other gene - editing tools such
as zinc -
finger nucleases (ZFN) and TAL effector
nucleases.
What's more, say the researchers, the cancer - causing effects of off - target deletions mistakenly created by the V (D) J enzyme need to be considered in designing site - specific enzymes for genome modification such
as zinc -
finger nucleases, TALENS, or CRISPRs.
In it, they sound the alarm about new genome - editing techniques known
as CRISPR and
zinc -
finger nucleases that make it much easier for scientists to delete, add, or change specific genes.
As an approach to inactivating CCR5, we introduced CCR5 - specific
zinc -
finger nucleases into human CD4 + T cells prior to adoptive transfer, but the need to protect cells from virus strains that use CXCR4 (X4) in place of or in addition to CCR5 (R5X4) remains.
Each ZFP was then fused to a modified FokI cleavage domain, active preferentially
as a dimer to reduce nonspecific DNA cleavage, resulting in
zinc -
finger nucleases (ZFNs)[25].
Ultimately this will include the expanding repertoire of advanced technologies such
as clustered regularly interspaced short palindromic repeats (CRISPR), CRISPR - associated protein 9 (Cas9), and
zinc -
finger nucleases (ZFNs)(46) along with the new clinical indications and markets that they address.