For the analysis of mRNA levels for
asparagine synthetase (ASNS), CHOP (CCAAT / enhancer - binding protein homologous protein), vascular endothelial growth factor (VEGF), glucose transporter 1 (Slc2a1) and monocarboxylate transporter 4 (Slc16a3), RT - PCR as well as qPCR were used.
«In healthy subjects, it seems that the level of
asparagine synthetase in the brain is sufficient to supply neurons,» Michaud said.
The team identified the gene affected by the mutation code for
asparagine synthetase, the enzyme responsible for synthesizing the amino acid asparagine.
Production of the enzyme
asparagine synthetase is deficient in ALL and several other types of cancer cells, which are therefore unable to synthesize the amino acid asparagine.