Sentences with phrase «assess cell proliferation»

Discrepancies between metabolic activity and DNA content as tool to assess cell proliferation in cancer research.

Immunohistochemical demonstration of Cyclin D1 have become an important mean for assessing cell proliferation, therefore the immunohistological demonstration of cell cycle related antigens such as CDKs which regulated by the different cyclins could provide a good tool for assessment of cellular proliferation [11].

Not exact matches

For (D)--(F), 5 × 104 Stemcell Technologies — purified naive CD8 + T cells were incubated with 0.3 μg / ml anti-CD3 Ab at 37 °C for 72 h. Cells were surface - stained for viability and evaluated for proliferation as assessed by dilution of CFSE within CD8 + - gated cellscells were incubated with 0.3 μg / ml anti-CD3 Ab at 37 °C for 72 h. Cells were surface - stained for viability and evaluated for proliferation as assessed by dilution of CFSE within CD8 + - gated cellsCells were surface - stained for viability and evaluated for proliferation as assessed by dilution of CFSE within CD8 + - gated cellscells (D).

Immunoblots showed almost complete inhibition of mTOR expression (as shown in Fig. 4B) that resulted in decreased cell proliferation as assessed by MTS assay (A).

Flow cytometric technique provides a method for assessing the cells nuclear DNA content and proliferation rate by using the value of S - phase fraction (SPF)[12].

To assess the sensitivity of other JAK1 mutation - positive cell lines to JAK inhibition, we performed a proliferation assay with increasing doses of INCB018424.

To assess the ability of F3 - IgG to inhibit the proliferation of HER2 - overexpressing breast carcinoma cells, we measured the proliferation of BT474, MCF7 / HER2 and MCF7 breast cancer cell lines in the presence of trastuzumab, F3 - IgG, or an isotype - matched control antibody.

Indeed, when we assessed these cells in suspension culture, overexpression of MELK significantly increased the proliferation of HMEC cells (Figure 4 — figure supplement 1A).

Intestinal permeability was assessed by Ussing chamber; epithelial cell (EC) ultra-structure by electron microscopy; RNA expression of genes coding for junctional proteins by Q - real - time PCR; immune response by in - vitro antigen - specific T - cell proliferation and cytokine analysis by cytometric bead array; intestinal microbiota by fluorescence in situ hybridization and analysis of systemic antibodies against intestinal microbiota by surface staining of live bacteria with serum followed by FACS analysis.

Professional Duties & Responsibilities Biomedical and biotechnology engineer with background in design of biomaterials, biosensors, drug delivery devices, microfrabrication, and tissue engineering Working knowledge of direct cell writing and rapid prototyping Experience fabricating nanocomposite hydrogel scaffolds Proficient in material analysis, mechanical, biochemical, and morphological testing of synthetic and biological materials Extensive experience in bio-imaging processes and procedures Specialized in mammalian, microbial, and viral cell culture Working knowledge of lab techniques and instruments including electrophoresis, chromatography, microscopy, spectroscopy, PCR, Flow cytometery, protein assay, DNA isolation techniques, polymer synthesis and characterization, and synthetic fiber production Developed strong knowledge of FDA, GLP, GMP, GCP, and GDP regulatory requirements Created biocompatible photocurable hydrogels for cell immobilization Formulated cell friendly prepolymer formulation Performed surface modification of nano - particle fillers to enhance their biocompatibility Evaluated cell and biomaterial interaction, cell growth, and proliferation Designed bench - top experiments and protocols to simulate in vivo situations Designed hydrogel based microfluidic prototypes for cell entrapment and cell culture utilizing computer - aided robotic dispenser Determined various mechanical, morphological, and transport properties of photocured hydrogels using Instron, FTIR, EDX, X-ray diffraction, DSC, TGA, and DMA Assessed biocompatibility of hydrogels and physiology of entrapped cells Evaluated intracellular and extracellular reactions of entrapped cells on spatial and temporal scales using optical, confocal, fluorescence, atomic force, and scanning electron microscopies Designed various biochemical assays Developed thermosensitive PET membranes for transdermal drug delivery application using Gamma radiation induced graft co-polymerization of N - isopropyl acylamide and Acrylic acid Characterized grafted co-polymer using various polymer characterization techniques Manipulated lower critical solution temperature of grafted thermosensitive co-polymer Loaded antibiotic on grafted co-polymer and determined drug release profile with temperature Determined biomechanical and biochemical properties of biological gels isolated from marine organisms Analyzed morphological and mechanical properties of metal coated yarns using SEM and Instron Performed analytical work on pharmaceutical formulations using gas and high performance liquid chromatography Performed market research and analysis for medical textile company Developed and implement comprehensive marketing and sales campaign