i bet that these time arsene will be ashamed of accepting a new contract after failing to win the pl or cl... 2 more seasons and arsenal will be free of captivity forever... with his attitude of wanting to prove the spenders wrong, he will remain a loser and i can bet my house that these guy won'
t win another major trophy
at arsenal... if he accepts another contract then he is A SHAMELESS OLD
T **
RT....
The membrane was blocked with 3 % BSA / PBS for 1 h
at RT followed by incubation with the corresponding primary antibody in 1 % BSA / PBS -
T overnight
at 4 °C: pTyr (Millipore, # 05 - 321, 1:500), Tie2 (R&D, #AF313, 1:1,000), Tie2 (R&D, #AF762), pAKT (Cell Signaling, # 4060S, 1:1,000), AKT (Cell Signaling, # 9272S, 1:1,000), FOXO1 (Cell Signaling, # 9454, 1:1,000), pFOXO3A (Cell Signaling, # 9466, 1:1,000), FOXO3A (Cell Signaling, # 12829, 1:1,000), VEGFR2 (Cell Signaling, # 2479, 1:1,000), tubulin (Sigma, #
T8203, 1:5,000).
Herman
T Salton, ««A Flag For All Republicans»: The Legislative History and Parliamentary Debates on the French Law on Religious Signs
at School» (2013) 2:1 Can J Hum
Rts 118.