Sentences with word «azide»
The word "azide" refers to a chemical compound that typically contains three nitrogen atoms bonded together. It is a very reactive and potentially explosive substance often used in explosives, detergents, and pharmaceuticals. Full definition
The cells were then washed once with FACS buffer I, once with FACS buffer II (0.01 % sodium azide in PBS), and fixed with 1 % formaldehyde in PBS.
biomedical-engineering-online.biomedcentral.com
Unlike Abdulmutallab's bomb, the new device contained lead azide, an explosive often used as a detonator.
The cells were enzymatically removed from the culture dish with Tryple ™ Select (Invitrogen) for 15 min at 37 °C, resuspended in 1 ml FACS buffer I (2 % FBS, 0.01 % sodium azide in PBS), and counted with Neubauer cell counter chambers.
The team points out that unclicking triazoles in this way opens up new ways of using their constituent azides and alkynes and might allow for the use of triazoles as mechanically labile protecting groups in synthetic reactions.
For example, triazoles could potentially be used to protect azides from harsh environments required to build another part of the same molecule.
Sections were stored in PBS containing 0,01 % Na - azide at 4 °C.
But making them involves sulfonyl azides and diazo compounds, which require very careful handling.
Genetic encoding and labeling of aliphatic azides and alkynes in recombinant proteins via a pyrrolysyl - tRNA synthetase / tRNA (CUA) pair and click chemistry.
Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide, and freeze - dried.
If the new underwear bomb had been used, the bomber would have ignited the lead azide, which would have triggered a more powerful explosive, possibly military - grade explosive pentaerythritol tetranitrate (PETN).
Shock -, light -, or heat - sensitive materials: Sodium azide — a common preservative in some buffers — comes to mind; it is sensitive to all three.
Sodium azide is / was the active ingredient in car emergency air bags — so the explosion potential is certainly present.
Lead azide and PETN were key components in a 2010 plan to detonate two bombs sent from Yemen and bound for Chicago — one in a cargo aircraft and the other in the cargo hold of a passenger aircraft.
These molecules are readily made using click chemistry by coupling an azide and alkyne, but the team reasoned the same triazole might be a useful protecting group in various chemical processes if there were a way to reverse the process.
After the desired reaction has taken place, mechanical force could then be used to liberate the azide, which can then be used for further chemical modification.
The antibody is provided as 100 uL of affinity purified serum in PBS (0.02 M sodium phosphate with 0.15 M sodium chloride, pH 7.5) with 1 % BSA (bovine serum albumin), and 0.02 % sodium azide.
Tissue section samples were thawed and permeabilized with PBS containing 0.2 % fish skin gelatin, 0.1 % Triton X-100 (PBS / FSG / TX) containing 0.02 % sodium azide (AZ) with gentle agitation for 1 hour.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Primary antibody incubated at 1/2000 for 2 hours @ 21 °C in TBS / BSA / azide.
Samples were loaded on a GE Superose 6 10/30 GL column in 0.15 M sodium chloride containing 1 mM ethylenediaminetetraacetic acid and 0.02 % sodium azide, pH 7.4.
In the case of Clearfield, the industrial compound sodium azide was used to induce mutations.
When the sensors detect a crash, they fire the air bag using the chemical sodium azide.
Ross Iannatti Hysteresis no. 62, 2016 Silicone coated nylon fabric, sodium azide, residue, wood 245 x 210 cm (96 x 47.25 inches)