Sentences with phrase «azide at»

Not exact matches

The cells were enzymatically removed from the culture dish with Tryple ™ Select (Invitrogen) for 15 min at 37 °C, resuspended in 1 ml FACS buffer I (2 % FBS, 0.01 % sodium azide in PBS), and counted with Neubauer cell counter chambers.

The brains were then transferred into a solution of 30 % w / v sucrose in PBS that was supplemented with 0.05 % sodium azide and stored at 4 °C until the brains had sunk to the bottom of the containers.

Primary antibody incubated at 1/2000 for 2 hours @ 21 °C in TBS / BSA / azide.