Sentences with phrase «bactrap transgenic mouse lines»

To this end, another PhD student in the lab, Ji - Feng Fei, generated a novel transgenic mouse line.

One of the most popular models is the immunodeficient nonobese diabetic severe combined immunodeficiency (NOD scid) gamma (NSG) transgenic mouse line, which can be endowed with a humanized immune system using CD34 + hematopoietic stem cells.

The transgenic mouse line APPswe / PS1ΔE9 85 was a generous gift of Dr. J.L. Jankowsky.

AβPP transgenic mouse lines differ in the types and amounts of Aβ that they generate and in their spatiotemporal patterns of expression of Aβ assemblies, providing a toolkit to study Aβ amyloidosis and the influence of Aβ aggregation on brain function.

For her dissertation, Prerana developed several bacTRAP transgenic mouse lines to characterize molecularly distinct cortical pyramidal cell types, and focused on layer 2/3 pyramidal cell population in the prefrontal cortex that moderate stress induced depression related behavior.

The mice were crossed with Slc6a14 − / − mice to generate these two transgenic mouse lines on Slc6a14 + / + and Slc6a14 − / − backgrounds.

The GENSAT database contains histological data from given BAC transgenic mouse lines at three developmental stages — embryonic day 15.5 (E15.5), postnatal day 7 (P7) and adult; in all cases the data represent results of multiple transgenic lines.

Not exact matches

A line of transgenic mice was studied that express the human LDL receptor gene in the liver under control of the transferrin promoter.

Human amyloid precursor protein (hAPP) transgenic mice from line J20, which carry mutations that cause early - onset AD (7), show major deficits in this assay (8).

Each of the endothelial cell lines established from the H - 2Kb - tsA58 transgenic mice was capable of generating vascular - like channels on Matrigel within 12 h.

I was able to establish a transgenic line from one of these founders (F66), and I backcrossed this line extensively to C57 BL / 6 mice for subsequent analysis.

Each of these four transgenic lines was backcrossed at least 6 times to C57 BL / 6 mice prior to analysis in order to control for genetic background effects.

EMMA comprises over 6000 mutant mouse lines carrying targeted, transgenic, endonuclease - mediated, induced and other types of mutations.

We measured Vc in axons of Schaffer collaterals in CA1 area of hippocampus in two transgenic mouse models of AD that over-express β - amyloid, line 41 (6mo) and APP / PS1 (24mo).

Sequencing of the mouse genome coupled with the successful development of transgenic and knockout mouse lines allows researchers to investigate the roles that individual genes play within specific mechanisms of renal physiology under both normal and pathologic conditions (14).

GMC strategy: To develop and offer a large scale standardised and comprehensive phenotypic analysis of mouse mutants from various sources (e.g. transgenic lines, knockout mice, ENU mutants or endonuclease mediated mutations)

We used 20 - month - old line 85 APPswe / PS1ΔE9 transgenic AD mice (huAPP / PS1) to examine the effect of J147 on the physiological, behavioral and pathological aspects of the disease when administered late in the course of the disease.

The APPswe / PS1ΔE9 transgenic mice (line 85) have been previously characterized [10, 11].

Research Focus: I am highly trained in producing and purifying monoclonal antibodies from Hybridoma cells, performing ELISA assays, preparing primary cells from organs and analyzing cell populations using FlowJo Cytometry data analysis software, isolating genomic DNA from animal tissues, maintaining and screening numerous transgenic and knock out mice lines by PCR and FACS.

There exist several dozen lines of transgenic mice that express human amyloid - β protein precursor (AβPP) with Alzheimer's disease (AD)- linked mutations.

provide a direct quantitative comparison of amyloid plaque burdens and plaque sizes in four lines of AβPP transgenic mice.

We found that the plaque burdens among the transgenic lines varied by an order of magnitude: at 15 months of age, the oldest age studied, the median cortical plaque burden in 5XFAD mice was already ∼ 4.5 times that of 21 - month - old Tg2576 mice and ∼ 15 times that of 21 — 24 - month - old rTg9191 mice.

In this study, we established mouse iPS cell lines from NPCs of neonatal EGFP - transgenic mice on the HFF feeder cells via viral transduction of 4 factors, combined with application of 2i.

Here, we... provide a direct quantitative comparison of amyloid plaque burdens and plaque sizes in four lines of AβPP transgenic mice.

These results are in line with recently published findings showing that in APP transgenic mice (Tg2576), the pesticide paraquat which enhances ROS levels also increases Aß levels and leads to cognitive impairment [36].

Given that many recent studies of B cell biology take advantage of Ig - transgenic (or knockin equivalent) mice for their entire analysis, we do not feel that our use of the approach as one part of the present study is out of line with currently accepted practice in the field.

For a period of five months they added GSPE to a standard diet and tested the mice for signs of memory deterioration normally found in this line of transgenic animals.