Sentences with phrase «bacterial genomes from»

When they sequenced the complete genomes of the Y. pestis DNA in those seven individuals, the team found that the bacterial genomes from the earliest samples lacked two genes that helped Y. pestis evade the immune systems of humans and fleas during the Black Death.

Craig Venter «s team at the J. Craig Venter Institute in Rockville, Maryland, and San Diego, California, has made a bacterial genome from smaller DNA subunits and then transplanted the whole thing into another cell.

The quest to create life in the lab took a leap forward in February when scientists at the J. Craig Venter Institute (JCVI) announced in Science [subscription required] that they had synthesized an entire bacterial genome from scratch.

Scientists today announced that they have crafted a bacterial genome from scratch, moving one step closer to creating entirely synthetic life forms — living cells designed and built by humans to carry out a diverse set of tasks ranging from manufacturing biofuels to sequestering carbon dioxide.

Not exact matches

The investigators examined 181 bacterial genomes taken from the female urinary microbiome, which Dr. Putonti said were representative of that microbiome's phylogenetic diversity.

Overlapping «cassettes» of 5 to 7 kilobases (kb), assembled from chemically synthesized oligonucleotides, were joined by in vitro recombination to produce intermediate assemblies of approximately 24 kb, 72 kb («1/8 genome»), and 144 kb («1/4 genome»), which were all cloned as bacterial artificial chromosomes in Escherichia coli.

Using a genome synthesized from chemical components, the researchers rebooted a bacterial cell to run on a new set of DNA instructions.

Last week, genomics pioneer Craig Venter announced that his team has passed an important milestone in its efforts to create a bacterial cell whose genome is entirely synthetic — constructed chemically from the building blocks of DNA.

But while Venter's synthetic genome will be housed within an existing bacterial cell, other scientists are aiming for the even more ambitious target of building an entire living cell from the basic chemical ingredients.

The genomes of these 377 bacterial isolates, plus an additional 107 single bacterial cells from roots of A. thaliana, were then sequenced, assembled, and annotated at the JGI.

The impetus for the meeting was a May report in Science in which researchers from the J. Craig Venter Institute synthesized the genome of a bacterium, added it to another bacterial cell, and got the cell to replicate using the new DNA.

Comas, too, is working on the evolution of bacterial genomes, but from a different perspective.

As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole genome as naked DNA.

The genes encoding NDM - 1 and other antibiotic resistance factors are usually carried on plasmids — circular strands of DNA separate from the bacterial genome — making it easier for them to spread through populations.

But Venter plans to scale up this process by combining many 5000 - base pieces to make larger genomes; IBEA's ultimate goal is to produce a small bacterial cell from scratch.

He says this idea has «very profound» implications for the debate over the origins of bacterial genes that are present in the human genome but absent in our closest relatives (Science, 8 June, p. 1903): The amount of conjugation Waters detected is «high enough to readily explain» the possible infiltration of bacterial genesinto our DNA, meaning that conjugation could have happened quickly enough to add genes only to humans, in the years since they split from the common ancestor they shared with chimpanzees.

The bacterial genomes come from species that challenge the technical performance of sequencing methods and have been determined by the Food and Drug Administration (link is external)(FDA) to have significant relevance to the research of public health issues such as food contamination, antibiotic resistance and hospital - acquired infections.

Zhao and colleagues examined the ability of the CRISPR - Cas system, a set of molecules borrowed from a form of immune system in bacteria (CRISPR stands for clustered regularly interspaced short palindromic repeats, describing a feature of this system in bacterial genomes).

During their coexistence, the bacterium was further and further integrated as a power plant into the cell, which was evident in the fact that the construction plans of the power plant were increasingly transferred into the host cell from the bacterial genome.

The first significant insight into the genome came from the analysis of 10 bacterial artificial chromosome (BAC) sequences (Kovach et al. 2010).

We have demonstrated the use of TFUMseq for high ‐ throughput in vivo screening of genetic fragments from an entire donor genome from a commensal microbe to increase the fitness of a phylogenetically distant bacterial species in the mammalian gut.

For instance, the Human Microbiome Project (HMP)(Turnbaugh et al, 2007; Peterson et al, 2009; Huttenhower et al, 2012) and MetaHIT (Qin et al, 2010) have generated maps of bacterial species abundances throughout the human body, reference genomes, and catalogs of more than 100 million microbial genes assembled from shotgun sequencing of in vivo communities.

$ 5,000 «A genome from your gut» Deep characterization of a single stool sample by shotgun metagenomics, includes best - effort attempt to assemble one or more bacterial genomes out of your gut.

Within a given bacterial species, gene content can vary by 50 % or more from the reference genome.

In their recent study, the team devised a way that ensures the memory element is not lost from the genome during the evolution of the bacterial population over more than a hundred generations.

To better understand the molecular drivers behind resistance, researchers at the Centers for Disease Control and Prevention (CDC) in Atlanta, Georgia, recently conducted a whole - genome analysis of an unusual bacterial strain cultured from a patient in the United States.

After this course you should be able to: - Prepare libraries from genomic DNA for amplicon or whole genome approaches to nanopore sequencing - Run ONT devices and assess sequencing performance during a run - Understand the basics of ONT data handling and analysis - Analyse and interpret ONT amplicon data and whole genomic data from bacterial samples

Bacterial Gene Numbers Vary from Approximately 500 to 8000 and Overlap Those of Single - Celled Eukaryotes The bacterial genome projects already provide excellent estimates for the number and types of protein and RNA molecules made by free living prokaryotes (Bacterial Gene Numbers Vary from Approximately 500 to 8000 and Overlap Those of Single - Celled Eukaryotes The bacterial genome projects already provide excellent estimates for the number and types of protein and RNA molecules made by free living prokaryotes (bacterial genome projects already provide excellent estimates for the number and types of protein and RNA molecules made by free living prokaryotes (Table 1).

Strains from the Olive (Olea europaea L.) Rhizosphere as Effective Biocontrol Agents against Verticillium dahliae: From the Host Roots to the Bacterial Genomes — Carmen Gómez - Lama Cabanás — Frontiers in Microbiofrom the Olive (Olea europaea L.) Rhizosphere as Effective Biocontrol Agents against Verticillium dahliae: From the Host Roots to the Bacterial Genomes — Carmen Gómez - Lama Cabanás — Frontiers in MicrobioFrom the Host Roots to the Bacterial Genomes — Carmen Gómez - Lama Cabanás — Frontiers in Microbiology

Her game - changing technology takes a mysterious bacterial genetic code and transforms it into a powerful tool for cutting and pasting bits of genetic material — meaning not only could the entire field of gene therapy be revived, but her genome - editing tool could one day be used to treat a range of diseases, from cancer and AIDS to hereditary disorders like Down syndrome and Huntington disease.

Then in 2005, another piece of the puzzle fell into place when researchers discovered that the short sequences between the repeats weren't from the bacteria themselves, but were actually viral DNA that had been added to the bacterial genome following an infection.

In this study we make use of the recently completed Danaus plexippus genome to identify a bacterial LGT of a glycosyl hydrolase from Enterococcus, show that the LGT is ancient in Lepidoptera, and for one species provide molecular evidence for its insertion into the genome.

To identity potential recent LGT events we used BLASTN to compare the D. plexippus genomic scaffolds against a bacterial database containing 1,097 complete bacterial genome sequences downloaded from the National Center for Biotechnology Information (NCBI).

Here, we performed whole - genome sequencing of paired isolates from the blood and CSF of 869 meningitis patients to determine whether such variation frequently occurs between these two niches in cases of bacterial meningitis.

Intracellular bacterial pathogens probably arose when their ancestor adapted from a free - living environment to an intracellular one, leading to clonal bacteria with smaller genomes and less sources of genetic plasticity.

The research, which was funded as part of a collaboration with the University of Oxford, involved professor Peter Andrew from the University of Leicester's Department of Infection, Immunity and Inflammation and professor Chris Bayliss from the University of Leicester's Department of Genetics and Genome Biology, the hepatobiliary and pancreatic surgeon Ashley Dennison from Leicester's Hospitals, the immunologist Luisa Martinez - Pomares from the University of Nottingham and the expert in bacterial pathogenesis Richard Moxon from the University of Oxford.

Many successful assembly applications of the Pacific Biosciences technology have been reported ranging from small bacterial genomes to large plant and animal genomes.