These findings encouraged us to explore the possibility of establishing a Cas9 / gRNA -
based gene modification platform for large animals.
Not exact matches
«New major
gene expression regulator in fungi: Researchers report prevalent DNA
base modification in the earliest fungal lineages.»
A significant advantage of ZFN
gene modification, compared to retrovirus
based approaches, is that only transient transgene expression is required to permanently engineer an HIV resistant cell.
Because of these limitations, the AAV approach is best suited for tissue culture
based approaches that require
modification of only a single allele of a
gene, such as the introduction of heterozygous endogenous epitope tags, or the introduction of dominant mutations of a
gene into the endogenous allele in cultured cells.
Therefore, applications that require simultaneous
modification of all alleles of a
gene, or which require modifying multiple
genes simultaneously in the same cell, are more appropriate for a CRISPR -
based approach.
These classifications are
based on large amounts of data inclusing clinical data, somatic mutations,
gene and alternative transcript expression, or structural DNA
modification, and involve high - dimensional statistical machine learning techniques.
Epigenetic
modification is certainly not the only CRISPR -
based technology designed to alter
gene expression.
Protocols for the
modification of BACs, BAC transgenesis production, and histology are available at the GENSAT website; a complete description of the procedure can be found in «A
gene expression atlas of the central nervous system
based on bacterial artificial chromosomes», Nature.
We furthermore show that several of these RNA
modifications have a dynamic response to environmental stress and that, in particular, changes in the tRNA wobble
base modification 5 - methoxycarbonylmethyl -2-thiouridine (mcm (5) s (2) U) lead to codon - biased
gene - expression changes in starved animals.