Many scientific journals require researchers who report results
based on cell line experiments to use DNA profiling to establish the identity of the used cells.
The resulting «map» of gene - drug interactions allowed the researchers to accurately predict the responses of multiple human cancer cell lines to different chemotherapy agents
based on the cell lines» genetic profiles and also revealed new genetic factors that appear to determine the response of breast and ovarian tumor cells to common classes of chemotherapy treatment.
Although the UCLA findings are preliminary,
based on cell lines, and more suggestive than bulletproof at this stage, the Reuters headline «Cancer Cells Slurp Up Fructose» is fair warning to any of us addicted to fruit and fruit juices.
Not exact matches
Research
based on such false
cell lines would undermine the understanding of different cancers and possible treatments, and clutter the scientific literature with bogus conclusions.
If the president's philosophy — to restrict federal funding to research
on cell lines created prior to August 9, 2001 — lacks a principled moral
basis that amplifies the view that the governmental obstacles imposed
on stem
cell research, and the delays they have caused, themselves represent an ethical problem.
In addition, the bank «will actively recruit» the holders of existing hES
cell lines around the world to deposit their
cells, Radda says, and will work out intellectual property agreements with
cell donors
on a case - by - case
basis.
Until the present study, demographic inference was
based on only three Aboriginal Australian genomes; one was derived from a tuft of hair (taken from a deceased individual), and the other two from
cell lines whose provenance is somewhat hazy.
They show that this new formulation reduces the minimal curative dose in a disease model,
based on infections in mice, by 100-fold and, most importantly, circumvents drug resistance in a
cell line that is resistant as a result of mutations in the transporter that mediates drug uptake.
The current study was
based on previous studies in the laboratory of Nita Ahuja, M.D., director of the Sarcoma and Peritoneal Surface Malignancy Program and professor of surgery at the Johns Hopkins University School of Medicine, which showed that guadecitabine limited the growth of colorectal cancer
cell lines when combined with irinotecan, says Azad.
The case for the all - conquering Africans is
based mainly
on studies of the Y chromosome and the mitochondrion, an energy - generating structure within the
cell that has its own small genome and passes down the female
line.
The potential influence of founder
cells is profound for regenerative medicine and other medical applications, since they suggest that the
cell line should be selected
based on the desired
cell type.
Roein - Peikar also noted that their experiments are mainly
based on Chinese Ovary Hamster
cells, a
cell line derived from the ovary of the Chinese hamster, often used in biological and medical research.
With so much work needed in studying the nature of stem
cells and using them to study disease processes, therapies
based on ES
cells seem very far down the
line, noted Lorenz Studer of Memorial Sloan - Kettering Cancer Center in New York, who pointed out that so far there have only been two published papers
on therapeutic cloning, both of them in mice.
Thus, our selection strategies, which were
based on targeting endothelial populations that expressed both of these inducible glycoproteins, suggest that the endothelial
cell lines generated in this study are most likely from this microcirculatory region.
\ n \ nIn other words this is a political attempt,
based on a technicality, to advance science in the field by getting the US Administration to greatly expand the number of human ES -
cell lines that can be used under federal funding.
The next step
based on these novel head and neck cancer discoveries, the scientists agree, is to tease out how the genes function in normal
cells, whether they form the
lining of the larynx, pharynx, or another anatomical site affected by head and neck cancer.
In the next decade, molecular research is going to further develop along five
lines: predictive medicine, that investigates the genetic conditions predisposing to tumor risk; early molecular diagnosis; the evaluation of each patient's prognosis
based on his / her genetic profile, in other words, the analysis of what kind of mutation affects the DNA of altered
cells; the investigation of the individual response to drugs,
based on our genetic knowledge; «smart drugs», molecules able to hit the target in a selective way, killing only the deprogrammed
cells.»
In MMEJ pathway, we achieve efficient gene disruption in human
cell lines and animals by developing a computer program that assists the choice of nuclease target sites
based on microhomology prediction.
The bottom
line, says Daley, is that research
on both types of stem
cells must continue, because it's too early to predict where the safest and most effective
cell -
based therapies will come from.
Based on mRNA expression data, two suitable
cell lines from the
cell line panel are selected for the immunofluorescent analysis of each protein.
The selection was furthermore
based on morphological characteristics, widespread use and multitude of publications using these
cell lines.
For a total number of 131
cell line samples and 172 tissue samples, mRNA sequencing was performed
on Illumina HiSeq2000 and 2500 machines (Illumina, San Diego, CA, USA) using the standard Illumina RNA - seq protocol with a read length of 2x100
bases.
Glial
cell line - derived neurotrophic factor (GDNF) was selected
based on clear evidence that it increases neuronal sprouting, prevents
cell death [20], [21] and has neuroprotective effects in the brain [11], [22]--[24], spinal cord [12] and retina [25]--[30], and because receptors for GDNF are expressed within mature retina [26], [31]--[34].
Based on the glycosylation targets of GalNAc - T6 in the cancer
cell lines, the authors hypothesized that expression of the enzyme disrupts epithelial development in the colon by affecting
cell -
cell adhesion.
We use an ES
cell line in which low levels of Hex transcript are visualized
based on the expression of the enhanced YFP, Venus coupled to a unique translational amplifier.
On the basis of studies on the XMRV - producing human prostate cancer cell lines CWR22Rν1 and CWR - R1 and their progenitor tumour xenograft CRW22, they concluded that XMRV infections were caused by contamination during in vivo passaging in nude mic
On the
basis of studies
on the XMRV - producing human prostate cancer cell lines CWR22Rν1 and CWR - R1 and their progenitor tumour xenograft CRW22, they concluded that XMRV infections were caused by contamination during in vivo passaging in nude mic
on the XMRV - producing human prostate cancer
cell lines CWR22Rν1 and CWR - R1 and their progenitor tumour xenograft CRW22, they concluded that XMRV infections were caused by contamination during in vivo passaging in nude mice.
That bill,
based on one from Rep. Diana DeGette, D - Colo., would have allowed federal dollars to flow toward research
on stem
cell lines using embryos left over from in vitro fertilization, slated for disposal and donated by the parents.
Other scalable systems include
cell lines based on the Henrietta Lacks tumor (HeLa) and baby hamster kidney (BHK - 21)
cells for adenovirus serotype 5 (Ad5) and herpes simplex virus 1 (HSV - 1) production (4, 40, 41).
Based on the resource of more than 47,000 antibodies and a panel of > 20 different human
cell lines, this facility has a unique position to investigate subcellular spatial proteomics for human and rodent biology.
Based on this analysis, it was mistakenly deduced that XMRV could not have originated from LNCaP or another
cell line in the laboratory.
Acute myeloid leukemia (AML) is the leading cause of leukemia mortality in the United States.1 Curative treatment involves intensive induction chemotherapy, before proceeding to either consolidation chemotherapy or allogeneic stem
cell transplantation
based on the patient's risk for relapse.2 This approach has been employed for > 4 decades and, although most individuals achieve complete remissions with front -
line therapy, 3 the majority of patients ultimately relapse with drug - resistant disease, and overall survival rates remain disappointingly poor.4 The limited ability of many patients to tolerate the intense chemotherapy -
based treatments, in particular hematological toxicity, further contributes to the poor outcomes noted in this disease.
One study examined the cytotoxic effects of different types of clove extract
on breast cancer
cell lines, including water -
based, alcohol -
based and essential oil extracts.
Year 6 Science Assessments and Tracking Objectives covered: Describe how living things are classified into broad groups according to common observable characteristics and
based on similarities and differences, including micro-organisms, plants and animals Give reasons for classifying plants and animals
based on specific characteristics Identify and name the main parts of the human circulatory system, and describe the functions of the heart, blood vessels and blood Recognise the impact of diet, exercise, drugs and lifestyle
on the way their bodies function Describe the ways in which nutrients and water are transported within animals, including humans Recognise that living things have changed over time and that fossils provide information about living things that inhabited the Earth millions of years ago Recognise that living things produce offspring of the same kind, but normally offspring vary and are not identical to their parents Identify how animals and plants are adapted to suit their environment in different ways and that adaptation may lead to evolution Recognise that light appears to travel in straight
lines Use the idea that light travels in straight
lines to explain that objects are seen because they give out or reflect light into the eye Explain that we see things because light travels from light sources to our eyes or from light sources to objects and then to our eyes Use the idea that light travels in straight
lines to explain why shadows have the same shape as the objects that cast them Associate the brightness of a lamp or the volume of a buzzer with the number and voltage of
cells used in the circuit Compare and give reasons for variations in how components function, including the brightness of bulbs, the loudness of buzzers and the
on / off position of switches Use recognised symbols when representing a simple circuit in a diagram
The world itself is fascinating too — it really is a fantasy
based on reality, and I loved seeing modern technology like
cell - phones, power -
lines and cars appear alongside fantastical weapons and gigantic summoned wizards.
Yes, there are places in the United States where the sun is likely to shine
on an un-obscured
basis far more often than in other places, and transmission via HVDC
lines (rather than AC) has materially less loss over long distances (and is convenient for solar since a solar
cell generates DC power in the first place; as such it requires only one conversion, to AC at the receiving end, if HVDC transmission is used.)