LN521
based stem cell cultures grow as monolayers on top of the laminin substrate and remain pluripotent without spontaneous differentiation.
Not exact matches
Culturing stem cells by day, and audiences in dingy stand - up clubs by night, it wasn't until Helen Pilcher left the lab that she started
basing her comedy on science.
A Freiburg -
based research group has developed a microfluidic chip where more than one hundred apidose - derived adult
stem cell cultures can grow and divide.
Differential osteogenicity of multiple donor - derived human mesenchymal
stem cells and osteoblasts in monolayer, scaffold -
based 3D
culture and in vivo.
As bioinformatics approaches can only provide partial solutions, a functional analysis pipeline,
based on primary (fibroblast)
cell cultures, induced Pluripotent
Stem cells (iPS) or simple model organisms, is truly essential.
Ultimately, we use all this information to establish innovative
culture techniques for generating unlimited neural
stem cell sources for the derivation of specific types of clinically relevant neuronal or glial
cells, for their use in disease modelling and drug discovery platforms, and towards refining their efficacy for future
cell replacement -
based therapy.
However, it is practically difficult to perform the rescue experiments in the organoid
culture system because the lentivirus transfection is required for the gene transduction into the primary
stem / progenitor
cells and the APC gene (> 8.5 k
base pairs) is too large to be cloned into the lentivirus.