Laboratory: Experience with laboratory applications in parasitology, immunology and molecular biology including, microscopy, BCA Protein Assay, ELISA, Flow Cytometry, Real Time qPCR, Luminex, Gas chromatography (GC) mass spectrometry, cotinine assays (saliva), magnetic
bead cell separation (MACS, Percoll and Ficoll) and whole blood cultures.
Cellular biology: Isolation and culture of various
cell types; cryopreservation; Mixed Lymphocyte Reaction (MLR); cloning by limiting dilution; cytotoxicity assays using Chromium - 51 release; proliferation assay (CFSE and 3H - thymidine); ELISA; Elispot; magnetic
beads separation.
To further characterize these
cells, we sorted the CD45 - expressing
cells by immunomagnetic
bead separation, and measured
cell purity by flow cytometry (Figure 1a — b).