In this approach, a promoterless reporter gene (for instance encoding
beta galactosidase, or beta geo) is introduced into ES cells.
In vitro, Pds has been shown to produce β - glucosidase, N - acetyl - β - glucosaminidase, acid and alkaline phosphatases, esterase / esterase lipase / lipase, leucine and vailine arylamidase, naphthol - AS - B1 - phophohydrolase, various proteinases (albumin / casein / gelatin), and urease, while no enzymatic activity was indicated for cystine arylamidase, α - chymotrypsin, alpha /
beta galactosidase, trypsin, β - glucoronidase, α - fucosidase, and α - mannosidase [11].
Selection for expression of the gene requires transcription from a cellular promoter, and consequently a mutation in a cellular gene, and the activity of the tagged gene can be followed by staining for
beta galactosidase activity.
In the picture depicted, one of the two copies of the gene of the mouse was replaced with the lac Z cassette expressing the bacterial
beta galactosidase and its activity in the kidney is followed by staining.
Not exact matches
Unflavoured: Protein Blend (73 %)(Pea Protein Isolate, Brown Rice Protein Concentrate, Hemp Protein), DigeZyme * ® * Patented Enzyme Complex (Protease, Amylase,
Beta - D -
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The
beta -
galactosidase messenger RNA splicing system provides a useful method for screening for splicing - defective mutations, several of which have been characterized.
Coupling of Tetrahymena ribosomal RNA splicing to
beta -
galactosidase expression in Escherichia coli
The Tetrahymena intervening sequence (IVS) has been inserted into the gene for the alpha - donor fragment of
beta -
galactosidase in a recombinant plasmid.
In the current test, these were the molecules p16, p21 and a positive test for
beta -
galactosidase activity.
This subtype, named by the authors «senescence - associated macrophages» or SAMs, share some biomarkers of senescent cells by expressing p16 (Ink4a) gene and an enzyme
beta -
galactosidase.
Our lead candidate, LYS - GM101, has been designed to replace this defective gene in the cells of GM1 patients, in order to allow for production of the functional lysosomal acid
beta -
galactosidase (bgal) enzyme.
In their terminally nonproliferative state both p21 + / + and p21 - / - cultures were positive for the senescence - associated
beta -
galactosidase (SA -
beta - gal) activity; in contrast, the labeling index of p21 + / + cells was low (< 5 %) whereas the labeling index of p21 - / - cells was high (> 30 %).
Comparative effects of exogenous lactase (
beta -
galactosidase) preparations on in vivo lactose digestion.
Corazza GR, Benati G, Sorge M, et al.
beta -
Galactosidase from Aspergillus niger in adult lactose malabsorption: a double - blind crossover study.
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