Sentences with phrase «biallelic gene knockout»

Here, we report that CRISPR / Cas9 mediated approach can efficiently induce monoallelic and biallelic gene knockout in goat primary fibroblasts.

Not exact matches

The single - gene knockout fibroblasts were successfully used for somatic cell nuclear transfer (SCNT) and resulted in live - born goats harboring biallelic mutations.

Using CRISPR / Cas9 technology, we generated biallelic knockout mutations in host genes encoding IRF5 and IL - 10RA in iPSCs, and confirmed their roles in limiting chlamydial infection in macrophages.