Sentences with phrase «binding affinity»

The degree of DNA methylation alters the binding affinity.

In addition, HIV - 1 transactivating protein (Tat) has a unique cysteine - rich region with zinc binding properties (Huang and Wang 1996) and has a high binding affinity for zinc (Frankel et al. 1988).

Yet most importantly, many (though not all) isoflavanones that have been tested have the same binding affinity as actual estrogen, but half the receptor - dependent transcriptional power.

Coumestol has the same binding affinity for the ERb receptor as estrogen, but it has much less of an affinity for ERa.

Androgens are taken up by prostate cells, where they either bind to the androgen receptor directly or are converted to dihydrotestosterone (DHT), which has a greater binding affinity for the androgen receptor than testosterone.

[15, 16] Coconut oil in the diet enhances insulin action and improves binding affinity compared to other oils.

Selenium also has a strong binding affinity for mercury and other heavy metals.

Don't forget that phytic acid has the strongest binding affinity to copper among all metal ions (Cu2 + > Zn2 + > Ni2 + > Co2 + > Mn2 + > Fe3 + > Ca2; see this review).

Aging - associated increases in oxidative damage to key enzymes results in their structural deformation and decreased binding affinity for the co-enzyme, causing a decrease in enzyme funtion.8 Ames» research has demonstrated that increasing the availability of acetyl L - carnitine and α - lipoic acid, two nutrients that serve as mitochondrial enzyme co-factors, restores the velocity of the reactions (KM) in the related enzymes, and thus restores aging mitochondria's ability to produce youthful levels of ATP.9 10 11 12 13 Part II focused on the inter-relationships among the folate, methylation and transsulfuration pathways, whose dysfunction results in increased free radical production coupled with disruption of glutathione (GSH) synthesis, thus accelerating mitochondrial decay and aging.

These NSC - secreted antibodies assemble properly, possess tumor cell - binding affinity and specificity, and can effectively inhibit the proliferation of HER2 - overexpressing breast cancer cells in vitro.

Every interaction that you build into the core contributes to additional binding affinity.»

Two independent reports have pointed the existence of a complex composed of STAT2 homodimers and IRF - 9 without STAT1 in IFNα - treated cells (32, 33), but this finding was tempered by the facts that their authors mentioned that this complex displayed only limited DNA - binding affinity for an ISRE sequence.

These modifications of p53 are believed to fine tune p53 - dependent gene regulation and cell fate decisions by affecting p53 protein stability, DNA binding affinity and interactions between p53 and other transcription cofactors.

To investigate whether or not somatic coding mutations contributed to the different antigenicity observed between clones, the authors undertook an in silico analysis of predicted MHC class I binding affinity for each allele of missense mutations.

Furthermore, in order to optimize the pharmacokinetic profile and to improve CXCR4 binding affinity of this class of molecules, dual pyrimidinyl compounds, substituted with different functional groups such as methoxy and morpholine, were synthesized (compounds 194 - 200).

Through this approach, she showed in mice that vaccine - induced immune responses — which correlated with predicted MHC binding affinity and the measured peptide - MHC complex stability — were associated with positive outcomes.

Introduction of an isobutyl or benzyl group at the α - carbon within the guanidine - methyl carbonyl group of compound 39 did not cause significant decrease of the binding affinity (compounds 42 and 43).

The most active derivatives of phenylguanides were characterized by the presence of aromatic groups on the main chain, have shown high binding affinity than the melamine derivative (THAM trisguanide), demonstrating the importance of the position of aromatic groups themselves.

An improved binding affinity was detected after the presence of long spacers, with lengths from 20 to 80Å, in a dimeric ligand suggesting two different binding sites [11, 82].

Results from binding affinity and Matrigel invasion assays (> 75 % at 10 nM) confirmed a CXCR4 antagonism potential for compound 185.

The process often involves a combination of many methods that exploit differences between various proteins in binding affinity, physico - chemical properties, size and biological activity.

He generated a series of mice, each of which carried a synaptotagmin with altered calcium - binding affinity.

Antibodies with a high target - binding affinity are known to be effective against cancer.

Because MHC alleles differ in their binding affinity for various peptides, a vaccine based on just one peptide may not work for everyone.

Traditionally, it has been considered that the more tightly a drug binds its target protein, i.e. the greater its binding affinity is, the more effective it will be.

By fusing that sequence to three growth factors they were able to increase their binding affinity by 2 - to 100-fold, which could reduce the need for higher doses in the future.

«I knew we were on the right track when phosphoarginine showed a strong binding affinity to ClpCP,» says Débora Broch Trentini, a postdoc in the Clausen lab and the first author of the Nature paper.

Phytic acid has a strong binding affinity to important minerals such as calcium, magnesium, iron, and zinc.

The team found that the white wines had low binding affinities, but all the reds bound readily: the tendency of 100 millilitres of red wine — about half a glass — to bind to PPAR - gamma is up to four times as strong as the same tendency in the daily dose of rosiglitazone (Food and Function, DOI: 10.1039 / c0fo00086h).

You need some active molecules, and this tool finds molecules that possess similar binding affinities to the same receptor.

In this study, PSMA binding affinities were assessed for three MR contrast agents, which were then imaged in living cells using a 9.4 tesla magnet MR system.

Not exact matches

Water binds all animate objects to the earth and is the source of affinity among living things, the ground of a profound, animistic empathy between humans and both animals and plants.

This approach was applied to the Shaker potassium channel and to a high - affinity peptide inhibitor (scorpion toxin) that binds to its pore entryway.

Extracted into a two - dimensional form, the novel material appears to have an affinity for binding with semiconductors like silicon and could make an efficient metal contact in two - dimensional electronic devices, the researchers said.

The binding sites are joined together by linkers that further enhance the affinity for PFOA.»

Because the mutation is within a protein that helps form the virus's structure, it may have something to do with binding — perhaps allowing the virus to bind to cells with greater affinity, Fauci says.

Shea notes that although he and his colleagues have yet to finalize their measurements on how well their nanoparticles bind to various PLA2 molecules, their test - tube results suggest that they could have a similar high affinity for PLA2s as their previous nanoparticles had for melittin, the bee venom protein that stopped the toxin in animal studies.

In Class II engagement, Sox9 binds to multiple active enhancer elements through sub-optimal, low - affinity Sox dimeric motifs (DNA sequences), resulting in a high level of transcription of cartilage - related genes.

«By measuring the oxygen - binding properties of these engineered hemoglobins, we discovered that the same individual mutations produced an increased oxygen - affinity in some combinations and they produced a decreased oxygen - affinity in other combinations.

If the core particle is more fully developed, bottom right, an affinity switch is triggered and the two chaperones are released to allow the regulatory particle to bind and form a proteasome.

After affinity maturation, two of the designed proteins, HB36 and HB80, bind H1 and H5 HAs with low nanomolar affinity.

Prof. You - Me Kim and her student Dajung Jung at Pohang University of Science and Technology (POSTECH), in collaboration with Prof. Wonhwa Cho's group at the University of Illinois at Chicago, have identified that the majority of human Src homology 2 (SH2) domains not only bind to proteins, but also interact with membrane lipids with high affinity and specificity.

For this study, 10 subjects with Alzheimer's underwent PET following the injection of three radiotracers: fluorine - 18 fluorodeoxyglucose (F - 18 FDG), which images regional metabolic activity; carbon - 11 Pittsburgh compound B (C - 11 PiB), which has an affinity for amyloid plaques; and F - 18 AV - 1451, an emerging imaging agent that binds to tau in the brain.

The closed form has a low affinity for MinD, i.e. the probability that it binds to MinD is quite small.

The pictures show that human Argonaute has a single binding site for GW182, and that binding of the guide sequence increases the affinity of the Argonaute and GW182.

The two binding sites give antibodies strong affinity for their targets, Shokat says, and he wanted to mimic that strategy by creating an inhibitor that bound to mTOR in two places.

In a painstaking series of some 10,000 experiments, he determined affinity of 35 different binding sites within the MHC clefts for the 20 naturally occurring amino acids that make up peptides.

Results showed that 29 patients» scans revealed strong - binding metastases with a high affinity for the imaging agent.

Thus, this study has implications for analysis of human vaccine studies, as in addition to searching for defined lineages it is worthwhile to perform functional analysis of monoclonal antibodies that may have found new structural solutions to high affinity binding which can not be discerned from DNA sequence alone.

This difference in binding ability to the two immunogens suggests the R2 immunogen may have driven the earlier stages of affinity maturation of this lineage in llama 1, which resulted in clones that could also recognize 96ZM651.02 gp140 as the immune response progressed and the lineage developed breadth via mutations in the V gene region.