Sentences with phrase «binding domain»

CEA, which segregates in several herding breeds with Collie ancestry, was mapped to a large region of CFA37 that included over 40 genes [92]; subsequently the fact that the disorder segregates in multiple, closely related breeds was used to reduce the size of the critical disease - associated region and pinpoint the causal mutation to a 7.8 kb intronic deletion in the NHEJ1 gene, which spans a highly conserved binding domain to which several developmentally important genes bind [91].

Estrogen non-responsive estrogen receptor alpha (ERalpha) knock - in (ENERKI) mice have a mutation (glycine 525 to leucine, G525L) in the ligand - binding domain of ERalpha.

Swapping the DNA binding domain of another telomeric protein, TRF2, with the zinc finger domains of TZAP showed that the TZAP zinc finger domains were sufficient to localize TRF2 to telomeres.

Plasmids from the paper are available at Addgene and include a variety of cMYC, KLF4, SOX2 and OCT4 DNA binding domain and transcription activation domain fusion constructs.

In the mouse model of type 1 diabetes, susceptibility was also associated with variation in CTLA - 4 gene splicing with reduced production of a splice form encoding a molecule lacking the CD80 / CD86 ligand - binding domain.

The lipid binding domain binds PtdIns (4,5) P2.

The XPA - binding domain of ERCC1 is required for nucleotide excision repair but not other DNA repair pathways

In a proof of principle study, we found that a previously undescribed single nucleotide polymorphism in the binding domain of the erythrocyte binding like protein (EBL) conferred a dramatic change in red blood cell invasion in mutant rodent malaria parasites Plasmodium yoelii.

Interferon regulatory factor subcellular localization is determined by a bipartite nuclear localization signal in the DNA - binding domain and interaction with cytoplasmic retention factors

Taken together, our results suggest that changes within the chaperone - binding domain of SptP in S Typhi hinder binding to its chaperone, resulting in instability, preventing translocation, and therefore restricting the intracellular activity of this effector.

Nuclear magnetic resonance structure of the nucleic acid - binding domain of severe acute respiratory syndrome coronavirus nonstructural protein 3.

We observed low repressive activity of all tested ETV6 variants, and variants located in the E26 transformation - specific binding domain (encoding p.A377T, p.Y401N) led to reduced binding to corepressors.

We further demonstrated that while S. Typhimurium SptP is stable intracellularly within S Typhi, S Typhi SptP is unstable, although stability could be recovered following replacement of the chaperone - binding domain with that of S. Typhimurium.

Once they'd found suitable pairs, they constructed three components: GBPa - VP16 (activation domain); GBPb - GAL4 (DNA - binding domain); and a UAS - driven luciferase reporter construct.

A Ca2 + binding domain has been identified at the extreme N - terminus that controls the strength of the interaction between PICK1 and GluA2.

We also solved the first ever structures of CDPKs (from plants or parasites) with both the kinase domain and the calcium - binding domain intact.

We replaced exon 3, which encodes a part of the RNA binding domain and is present in all Boule isoforms (Figure 3C), with a lacZ - neo vector through homologous recombination in embryonic stem cells.

The removal of exon 3 resulted in a deletion of the RNA binding domain and a frame - shift in the remaining transcript.

This transcript is expected to produce a truncated BOULE protein missing both its RNA binding domain and the remaining C - terminal portion of the protein.

(A) Alignment of Boule RRM domains from representative species of both deuterostomes and protostomes reveals conservation of the RNA binding domain, in particular in the regions surrounding RNP2 and RNP1 as well as the C - terminal region.

The Eph receptor domains are indicated on the left; LBD, ligand - binding domain.

In this protein, the binding domain of botulinum toxin is replaced to broaden the toxin's therapeutic potential and allow drug development to be targeted towards a specific disease.

In contrast, the retrograde transport of the Shiga toxin B subunit between the plasma membrane and the Golgi complex is partly inhibited in cells overexpressing the Rab6 - binding domain of Rab6IP2.

About 20 years ago, it was discovered that the unique immune systems of camelids (llamas, camels, and alpacas) produce a pared down version of antibodies, featuring a single binding domain called a nanobody.

Relocation of a PIP3 binding domain to the plasma membrane of dictyostelium cells in response to cAMP.

We used this DNA binding domain to design a synthetic promoter (PlantPho) and modified PhoB with a eukaryotic transcriptional activator, VP64 [12].

In 2002, his group was the first to report the design of «second - generation» CARs that, in addition to a binding domain outside of the T cell and a signaling domain inside, included a costimulatory domain designed to promote cell proliferation and survival.

PhoB has a well characterized DNA binding domain whose affinity for DNA is strongly enhanced by phosphorylation [17], [18], [19].

Rapamycin forms an inhibitory complex with the FKBP - rapamycin - binding domain of mTOR, which probably destabilizes the mTOR - raptor interaction and prevents mTOR from phosphorylating p70S6K and 4E - BP1 (18, 19).

It has three key protein domains: a DNA - binding domain, a chromodomain (which may bind euchromatin), and a helicase domain (which is thought to activate transcription by acting against repressing transcription effects, such as heterochromatin structure).

The exomer cargo adaptor structure reveals a novel GTPase - binding domain.

Aurora A regulates the activity of HURP by controlling the accessibility of its microtubule - binding domain.

The Scw1 RNA - binding domain protein regulates septation and cell - wall structure in fission yeast.

Their experiments also revealed in detail how one region of the p53 protein, called the DNA - binding domain, serves double duty in the machinery.

The Adipophilin C Terminus Is a Self - folding Membrane - binding Domain That Is Important for Milk Lipid Secretion.

Dr. June: I mentioned that CAR is a chimeric molecule that has both the binding domain made of an antibody and a signaling engine that's inside the cell.

They found that the interaction of the C3dg fragments on affected erythrocytes and the binding domain for CR3 on immune cells can lead to phagocytosis and the rupturing of red blood cells in PNH patients.

Active genes are found in and around the CenH3 - binding domain.

(b) Human neocentromere 10q25.3 contains an approximately 330 kb CenH3 - binding domain contained in an approximately 700 kb region that can be precipitated with CREST # 6 antibodies and is flanked by late - replicating regions.

But because the CenH3 - binding domain has not been determined for other rice centromeres, the possibility that active genes and frequent retrotransposon insertions are a common feature of grass centromeres can not yet be ruled out.

The VHH is the total antigen binding domain or active site of these single - headed immunglobulins (IgG) and llama antibodies have been studied in this single domain form due to the dual advantages of bacterial / yeast expression and their high levels of thermal and pH stability [28].

We also identify a host protein called methyl - CpG binding domain protein 2 (MBD2) that binds methylated HIV DNA and is an important mediator of latency.

Cloning and sequencing of complementary DNA's from normal and WHHL rabbits, shows that this defect arises from an in - frame deletion of 12 nucleotides that eliminates four amino acids from the cysteine - rich ligand binding domain of the LDL receptor.

The RNA binding domain (TRBD) of telomerase is a crucial component to this process and, therefore, the enzyme's ability to work.

The research, led by Jeremy Smith, a Governor's Chair at the University of Tennessee (UT) and director of the UT - ORNL Center for Molecular Biophysics, revealed in atomistic detail why lignin is such a problem: Not only does it bind to cellulose in the preferred locations sought by enzymes, but lignin also attracts and occupies the cellulose - binding domain of the enzymes themselves.

Even though the 31mer encompassed the sequence encoding the putative helix - coil - helix - binding domain, it was not sufficient for binding to the 26 - base pair DNA crossover site.

In order to define the DNA - binding domain of the Hin protein two peptides 31 and 52 amino acids long were synthesized.

They retained, for example, the amino acid residues that bind to G - tract RNA, and the researchers confirmed that, like their mammalian counterparts, each RNA - binding domain of Glo can bind to this type of RNA sequence.

The crystal structures of hexameric DnaB and its complex with the helicase binding domain (HBD) of DnaG reveal that within the hexamer the two domains of DnaB pack with strikingly different symmetries to form a distinct two - layered ring structure.

This structure places all four DNA binding domains of intact LacR on the same side of the tetramer, and results in a deep, V - shaped cleft between the two dimers.