Sentences with phrase «binucleate hepatocytes»

They transplanted the hepatocyte - like cells into mice; 14 days later, some of the corrected cells had integrated into the rodent liver and were able to produce human A1AT.

Gandhi's research team is the first to develop a mouse model with depleted levels of a protein called augmenter of liver regeneration (ALR), which is essential for the survival of liver cells called hepatocytes.

Further experimentation showed Lagasse that if he injected hepatocytes directly into the lymph nodes, the cells picked up signaling proteins (essentially SOS signals to grow) released into the bloodstream from the dying liver.

The scientists started with metabolically relevant cells — hepatocytes and adipocytes — representing major functions of the liver and adipose tissues, and thus important aspects of the body's energy processing and storing system.

Hepatocytes (liver cells) have the highest concentration of it - up to 8 % of the fresh weight in well fed state, or 100 - 120 g in an adult.

To address this, Lagasse has injected hepatocytes into lymph nodes in peripheral parts of a mouse's body — under the knee or arm — because that requires less invasive surgery.

Rejection is not a problem because the animals were genetically engineered to share identical DNA, eliminating the risk that the immune system would attack foreign hepatocytes.

They found that hepatitis in these «human hepatocyte chimeric mice» was caused by white blood cells known as cytotoxic T lymphocytes (CTLs) that were specifically targeted to hepatitis B virus.

«We think the combination of making more hepatocytes and reducing collagen is the most promising approach to treating liver fibrosis,» says Willenbring.

Once inside the myofibroblasts, the virus downloads the transcription factors, which transform the cells into hepatocytes.

Liver failure occurs when healthy cells called hepatocytes are damaged by alcohol and disease.

Hepatocyte - specific Sqle transgenic expression in mice accelerated the development of high - fat, high - cholesterol diet — induced HCC.

Immunohistochemical stainings of Ki67 and pancytokeratin (Ki67 is stained brown, pancytokeratin is stained pink) indicate proliferation of hepatocytes (arrowheads) and biliary epithelial cells (arrows) in TAK1 / RIP3 - deficient mice.

In the course of the inflammatory process, the liver cells (hepatocytes) die more frequently due to programmed cell death.

To validate their computer modeling predictions, researchers performed experiments in human cancer cell lines, mouse liver samples and primary human hepatocytes.

To prove this point, he and Saito applied their miRNA switches to the purification of hepatocytes and pancreatic cells, neither of which has unique cell surface markers, finding miRNA switches effective there too.

The researchers then tested the particles» ability to shut off the gene for a blood clotting protein called Factor VII, which is produced in the liver by cells called hepatocytes.

Although the particles did penetrate endothelial cells in the liver, they did not enter liver hepatocytes.

Anderson and Langer have previously developed nanoparticles, now in clinical development, that can deliver siRNA to liver cells called hepatocytes by coating the nucleic acids in fatty materials called lipidoids.

«Growing primary cells like hepatocytes from a human is harder,» he says.

However, Mori confirmed in an e-mail that the problematic publication is «Downregulation of citrin, a mitochondrial AGC, is associated with apoptosis of hepatocytes,» which appeared in the 28 December 2007 issue of Biochemical and Biophysical Research Communications.

This colored scanning electron micrograph shows several specialized elements within a liver cell (hepatocyte).

The host restriction factor Smc6 (green) is located within the nucleus (blue) of uninfected human hepatocytes.

But while researchers have previously been able to infect cultures of human hepatocytes with HBV, the cells» limited lifespan has made it difficult to study the virus, says Bhatia, who is also a Howard Hughes Medical Institute investigator and a member of MIT's Koch Institute for Integrative Cancer Research and Institute for Medical Engineering and Science.

The hepatocytes are first patterned onto surfaces dotted with tiny spots of collagen, and then surrounded by supportive tissue made up of stromal cells, which act as connective tissue and support the hepatocytes in carrying out their liver functions.

In contrast, Smc6 is not present in HBV - infected hepatocytes (red).

They based their approach on a system they had previously developed for studying the hepatitis C virus, in which they were able to successfully infect human hepatocytes with the virus and use it to compare antiviral regimens.

One uses primary hepatocytes obtained from livers donated for transplant; the second uses stem cells derived from human skin samples and guided into hepatocyte - like cells, Bhatia says.

«That's because the hepatocyte — the main cell in the liver — is unstable,» she says.

However, unlike the primary hepatocytes, the hepatocyte - like cells offer an unlimited supply of test cells, since the researchers can simply grow more as required, Bhatia says.

It enters into normal hepatocytes to keep them skinny, which prevents the buildup of fat inside cells seen in steatosis, whereas it accumulates on the surface of HCC cells to kill them,» says Professor Miyazaki.

This involved culturing two liver cells (hepatocytes) that can act as a functional organ unit on artificial membranes fabricated with microwell patterns.

«Investigators have been developing in - vitro liver models for 40 years, but all of those systems ignore the distinct patterns of metabolically active hepatocytes that exist within the liver sinusoid» says Martin Yarmush, MD, PhD, director of the MGH - CEM and the paper's senior author.

They found that the starvation - induced accumulation of p53 was indeed detectable in hepatocytes, irrespective of whether these cells were of mouse or human origin.

For example, if blood - sugar - lowering insulin is fed into one of the device's two inlets while glucagon, which raises blood sugar, is added through the other, the metabolism of the hepatocytes is changed so that those on one side release glucose while those on the other take it up.

From one end of the sinusoid to another, the hepatocytes have different metabolic functions, often controlled by external factors and gene expression.

In order to determine which type of liver cells cause this accumulation, the researchers repeated the experiment using cultured hepatocytes.

Without CPEB4, the endoplasmic reticulum is unable to activate the stress response, thus causing hepatocytes to accumulate the lipids produced by the fatty liver.

«Liver tissue model accurately replicates hepatocyte metabolism, response to toxins: Microfluidic device may help predict toxic effects of new drugs, study liver disease.»

In order to develop a system that more closely replicates the metabolic differences among hepatocytes, the research team developed a microfluidic device that distributes hormones or other chemical agents across a 20 - to 40 - cell - wide sample of hepatocytes in such a way that the effects on the liver cells vary from one side to the other.

The use of other agents produced similar results across the field of hepatocytes regarding nitrogen metabolism or alcohol degradation, and use of a molecule that induces the expression of drug metabolism enzymes resulted in varied zones of susceptibility to the toxic effects of acetaminophen.

In this schematic of the new «liver on a chip» device, adding blood - sugar - raising glucagon to inlet 1 and blood - sugar - lowering insulin to inlet 2 distributes the hormones across the field of hepatocytes (purple box) along opposing gradients, altering the cells» metabolism from glucose release on the left side to glucose uptake on the right.

When blood passes through the liver, it travels from arteries to veins through channels called sinusoids, lined with the liver cells called hepatocytes.

«As mature hepatocytes die off, these clones replace the liver mass.

Non-alcoholic fatty liver is characterised by the accumulation of fat deposits in hepatocytes.

The research also detected the proliferation of binucleate hepatocytes a sign that the liver is attempting to rectify damage caused by a low protein diet.

«They are not doing all the «worker bee» functions of normal hepatocytes.»

The liver's cells, called hepatocytes, work to filter and remove toxins from the blood.

When Lin engineered the telomerase - expressing hepatocytes to die in response to a chemical signal and gave the mice with a liver - damaging chemical, he found that those animals in which the telomerase cells had been killed exhibited much more severe liver scarring than those in which the cells were functional.

Many conventional hepatocyte cells that are transplanted to mice for in vivo testing last for only two or three days, but the drug and its various metabolites might take weeks to metabolize, so toxic effects might not be apparent in such testing.