siTRF2 transfected cells were treated with increasing concentrations of
Bleomycin sulphate (0µM, 40µM and 80µM) and immunocytochemistry was performed as per laboratory standardized protocols [22].
Comparative DNA damage profile and SP enrichment post
Bleomycin sulphate treatment.
c) Graph showing dose dependant (20, 40, 60, 80 µM) increase in CD44 enriched cells post treatment with
Bleomycin sulphate (p < 0.001).
d) Immunocytochemistry analysis showing reduced CD44 expression in siTRF2 silenced HCT116 post treatment with
Bleomycin sulphate when compared to scrambled transfected HCT116 post treatment with Bleomycin.