Sentences with phrase «blocking reagent»
The sections were treated with 3 % H2O2 in phosphate buffer saline (PBS), then incubated with serum blocking reagent for 30 minutes.
http://www.oncm.org/
To block endogenous biotin, slides were first incubated in Avidin blocking solution followed by Biotin blocking reagent (Vector Laboratories, Burlingame, CA) for 15 minutes each at 37 ˚C.
Slides were then incubated in peroxidase blocking reagent (ready to use reagent, K1500, DAKO) for 5 min at room temperature.
Normal serums are recommended as a blocking reagent to reduce background from non-specific, conserved sequence and / or Fc - receptor binding.
Not exact matches
The team tested reagents that block the intracellular signals triggered by TSLP in NH cells.
HGS, he claims, is ready to share data and reagents with them: «We would not block anyone in the academic world from using this for research purposes.»
The slides were incubated with the primary antibody overnight at 4 °C, rinsed three times with PBS, incubated for 10 min in protein blocking solution, and incubated with the appropriate secondary reagent.
R&D Systems offers a wide range of reagents and kits designed to study the immune checkpoint, including blocking antibodies, proteins, assays, and more.